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Transient DNA Occupancy of the SMC Interarm Space in Prokaryotic Condensin.
- Source :
-
Molecular cell [Mol Cell] 2019 Jul 25; Vol. 75 (2), pp. 209-223.e6. Date of Electronic Publication: 2019 Jun 11. - Publication Year :
- 2019
-
Abstract
- Multi-subunit SMC ATPases control chromosome superstructure and DNA topology, presumably by DNA translocation and loop extrusion. Chromosomal DNA is entrapped within the tripartite SMCkleisin ring. Juxtaposed SMC heads ("J heads") or engaged SMC heads ("E heads") split the SMCkleisin ring into "S" and "K" sub-compartments. Here, we map a DNA-binding interface to the S compartment of E heads SmcScpAB and show that head-DNA association is essential for efficient DNA translocation and for traversing highly transcribed genes in Bacillus subtilis. We demonstrate that in J heads, SmcScpAB chromosomal DNA resides in the K compartment but is absent from the S compartment. Our results imply that the DNA occupancy of the S compartment changes during the ATP hydrolysis cycle. We propose that DNA translocation involves DNA entry into and exit out of the S compartment, possibly by DNA transfer between compartments and DNA segment capture.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Adenosine Triphosphatases genetics
Adenosine Triphosphate genetics
Bacterial Proteins chemistry
Cell Cycle Proteins chemistry
Chromosomes, Bacterial genetics
DNA chemistry
DNA-Binding Proteins chemistry
Hydrolysis
Multiprotein Complexes genetics
Nucleic Acid Conformation
Prokaryotic Cells chemistry
Bacillus subtilis genetics
Bacterial Proteins genetics
Cell Cycle Proteins genetics
DNA genetics
DNA-Binding Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4164
- Volume :
- 75
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular cell
- Publication Type :
- Academic Journal
- Accession number :
- 31201090
- Full Text :
- https://doi.org/10.1016/j.molcel.2019.05.001