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Inhibition of Kv2.1 Potassium Channels by MiDCA1, A Pre-Synaptically Active PLA 2 -Type Toxin from Micrurus dumerilii carinicauda Coral Snake Venom.
- Source :
-
Toxins [Toxins (Basel)] 2019 Jun 12; Vol. 11 (6). Date of Electronic Publication: 2019 Jun 12. - Publication Year :
- 2019
-
Abstract
- MiDCA1, a phospholipase A <subscript>2</subscript> (PLA <subscript>2</subscript> ) neurotoxin isolated from Micrurus dumerilii carinicauda coral snake venom, inhibited a major component of voltage-activated potassium (Kv) currents (41 ± 3% inhibition with 1 μM toxin) in mouse cultured dorsal root ganglion (DRG) neurons. In addition, the selective Kv2.1 channel blocker guangxitoxin (GxTx-1E) and MiDCA1 competitively inhibited the outward potassium current in DRG neurons. MiDCA1 (1 µM) reversibly inhibited the Kv2.1 current by 55 ± 8.9% in a Xenopus oocyte heterologous system. The toxin showed selectivity for Kv2.1 channels over all the other Kv channels tested in this study. We propose that Kv2.1 channel blockade by MiDCA1 underlies the toxin's action on acetylcholine release at mammalian neuromuscular junctions.
- Subjects :
- Animals
Cells, Cultured
Ganglia, Spinal cytology
Ganglia, Spinal physiology
Kv1.2 Potassium Channel genetics
Kv1.2 Potassium Channel physiology
Male
Mice, Inbred C57BL
Neurons drug effects
Neurons physiology
Oocytes physiology
Phospholipases A2
Xenopus
Coral Snakes
Elapid Venoms toxicity
Kv1.2 Potassium Channel antagonists & inhibitors
Potassium Channel Blockers toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 2072-6651
- Volume :
- 11
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Toxins
- Publication Type :
- Academic Journal
- Accession number :
- 31212818
- Full Text :
- https://doi.org/10.3390/toxins11060335