Reactive oxygen species altering the metabolite profile of the marine-derived fungus Dichotomomyces cejpii F31-1.

Authors :: Shaker S
Sun TT
Wang LY
Ma WZ
Wu DL
Guo YW
Dong J
Chen YX
Zhu LP
Yang DP
Li HJ
Lan WJ
Source :: Natural product research [Nat Prod Res] 2021 Jan; Vol. 35 (1), pp. 41-48. Date of Electronic Publication: 2019 Jun 19.
Publication Year :: 2021
Abstract: To investigate the influence of reactive oxygen species (ROS) on the secondary metabolites of the marine-derived fungus Dichotomomyces cejpii F31-1, hydrogen peroxide (H <subscript>2</subscript> O <subscript>2</subscript> ) was added to the GPY culture medium. The HPLC chromatogram of the EtOAc extract of the culture broth was distinct from that of the H <subscript>2</subscript> O <subscript>2</subscript> free GPY medium. Further study of the metabolites in the GPY medium with H <subscript>2</subscript> O <subscript>2</subscript> resulted in the discovery of eight known compounds. Among them, (22 E )-5α, 8α-epidioxyergosta-6, 22-dien-3 β -ol ( 2 ) and ergosta-4,6,8(14),22-tetraene-3-one ( 3 ) were present in the highest concentration, while ergosterol and diketopiperazines are abundant in the H <subscript>2</subscript> O <subscript>2</subscript> free medium. Additionally, a new compound, dichocetide D ( 1 ) containing a chlorine element and a known ergosterol ( 10 ) were isolated from the H <subscript>2</subscript> O <subscript>2</subscript> free medium. (22 E )-5α, 8α-epidioxyergosta-6, 22-dien-3 β -ol ( 2 ) exhibited moderate cytotoxic activity against human prostate cancer cell line LNCaP-C4-2B.

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