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Repetitive high-frequency transcranial magnetic stimulation reverses depressive-like behaviors and protein expression at hippocampal synapses in chronic unpredictable stress-treated rats by enhancing endocannabinoid signaling.
- Source :
-
Pharmacology, biochemistry, and behavior [Pharmacol Biochem Behav] 2019 Sep; Vol. 184, pp. 172738. Date of Electronic Publication: 2019 Jun 20. - Publication Year :
- 2019
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Abstract
- The anti-depressant effect of repetitive transcranial magnetic stimulation (rTMS), a clinically-useful treatment for depression, is associated with changes to the endocannabinoid system (ECS). However, it is currently unknown whether different frequencies of rTMS alter the ECS differently. To test this, rats exposed to chronic unpredictable stress (CUS) were treated with rTMS at two different frequencies (5 (high) or 1 Hz (low), 1.26 Tesla) for 7 consecutive days. Twenty-four hours after the final rTMS treatment, we evaluated depressive-like behaviors and the expression of several synaptic proteins and ECS-related proteins in the hippocampus. In addition, we knocked-down diacylglycerol lipase alpha (DAGLα) and cannabinoid type 1 receptor (CB1R), two important components of the ECS, and measured depressive-like behaviors and synaptic protein expression following rTMS. Furthermore, we measured the expression levels of several components of the ECS system in hippocampal-derived astrocytes and neurons exposed to repetitive magnetic stimulation (rMS) with different parameters (5 or 1 Hz, 0.84 or 1.26 Tesla). Interestingly, we found that only high-frequency rTMS ameliorated depressive-like behaviors and normalized the expression of hippocampal synaptic proteins in CUS-treated rats; this effect was eliminated by knockdown of DAGLα or CB1R. Moreover, we found that rMS at 5 Hz increased the expression of DAGLα and CB1R in hippocampal astrocytes and neurons. Collectively, our results suggest that high-frequency rTMS exerts its anti-depressant effect by up-regulating DAGLα and CB1R.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Female
Male
Pregnancy
Rats
Animals, Newborn
Astrocytes metabolism
Behavior, Animal
Gene Expression Regulation
Gene Knockdown Techniques
Neurons metabolism
Rats, Sprague-Dawley
Depression therapy
Endocannabinoids genetics
Endocannabinoids metabolism
Hippocampus metabolism
Lipoprotein Lipase genetics
Lipoprotein Lipase metabolism
Receptor, Cannabinoid, CB1 genetics
Receptor, Cannabinoid, CB1 metabolism
Stress, Physiological
Synapses metabolism
Transcranial Magnetic Stimulation methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-5177
- Volume :
- 184
- Database :
- MEDLINE
- Journal :
- Pharmacology, biochemistry, and behavior
- Publication Type :
- Academic Journal
- Accession number :
- 31229467
- Full Text :
- https://doi.org/10.1016/j.pbb.2019.172738