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Integration of renewable deep eutectic solvents with engineered biomass to achieve a closed-loop biorefinery.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2019 Jul 09; Vol. 116 (28), pp. 13816-13824. Date of Electronic Publication: 2019 Jun 24. - Publication Year :
- 2019
-
Abstract
- Despite the enormous potential shown by recent biorefineries, the current bioeconomy still encounters multifaceted challenges. To develop a sustainable biorefinery in the future, multidisciplinary research will be essential to tackle technical difficulties. Herein, we leveraged a known plant genetic engineering approach that results in aldehyde-rich lignin via down-regulation of cinnamyl alcohol dehydrogenase ( CAD ) and disruption of monolignol biosynthesis. We also report on renewable deep eutectic solvents (DESs) synthesized from phenolic aldehydes that can be obtained from CAD mutant biomass. The transgenic Arabidopsis thaliana CAD mutant was pretreated with the DESs and showed a twofold increase in the yield of fermentable sugars compared with wild type (WT) upon enzymatic saccharification. Integrated use of low-recalcitrance engineered biomass, characterized by its aldehyde-type lignin subunits, in combination with a DES-based pretreatment, was found to be an effective approach for producing a high yield of sugars typically used for cellulosic biofuels and biobased chemicals. This study demonstrates that integration of renewable DES with plant genetic engineering is a promising strategy in developing a closed-loop process.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Alcohol Oxidoreductases chemistry
Alcohol Oxidoreductases genetics
Aldehydes chemistry
Aldehydes metabolism
Arabidopsis metabolism
Biomass
Interdisciplinary Research
Lignin chemistry
Lignin genetics
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Solvents chemistry
Arabidopsis genetics
Biofuels
Genetic Engineering
Lignin biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1091-6490
- Volume :
- 116
- Issue :
- 28
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 31235605
- Full Text :
- https://doi.org/10.1073/pnas.1904636116