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Rice protein reduces DNA damage by activating the p53 pathway and stimulating endogenous antioxidant response in growing and adult rats.
- Source :
-
Journal of the science of food and agriculture [J Sci Food Agric] 2019 Oct; Vol. 99 (13), pp. 6097-6107. Date of Electronic Publication: 2019 Jul 29. - Publication Year :
- 2019
-
Abstract
- Background: Reactive oxygen species (ROS) can cause DNA damage. Rice protein (RP) inhibits ROS accumulation. However, a link between the reduction of ROS-derived DNA damage and the intake of RP is far from clear. The main objective of this study is to elucidate the effects of RPs on the reduction of DNA damage in growing and adult rats.<br />Results: An intake of RP for 2 weeks significantly reduced the hepatic accumulation of ROS and 8-hydroxydeoxyguanosine (8-OHdG) in growing and adult rats, whereas the hepatic p53 content was markedly increased by RPs. After 2 weeks' feeding, the mRNA levels and protein expressions of p53, ataxia-telangiectasia mutated (ATM), and Checkpoint kinase 2 (Chk2) were up-regulated by RPs, whereas Murine Double Minute 2 (MDM2) expressions were markedly inhibited by RPs, resulting in more p53 being translocated into the nucleus. Nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2) was activated by RP by reducing Kelch-like ECH-associated protein 1 (Keap1), resulting in the up-regulation of antioxidant expressions of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in RP groups.<br />Conclusion: Rice protein can exert an endogenous antioxidant activity to reduce ROS-derived DNA damage by activating the Nrf2-Keap1 pathway. This study suggests that the activation of the ATM-Chk2-p53 pathway might be one of the mechanisms exerted by RP for reducing DNA damage in growing and adult rats. © 2019 Society of Chemical Industry.<br /> (© 2019 Society of Chemical Industry.)
- Subjects :
- Animals
Catalase genetics
Catalase metabolism
Checkpoint Kinase 2 genetics
Checkpoint Kinase 2 metabolism
Glutathione metabolism
Glutathione Peroxidase genetics
Glutathione Peroxidase metabolism
Kelch-Like ECH-Associated Protein 1 genetics
Kelch-Like ECH-Associated Protein 1 metabolism
Liver metabolism
Male
NF-E2-Related Factor 2 genetics
NF-E2-Related Factor 2 metabolism
Oxidative Stress
Proto-Oncogene Proteins c-mdm2 genetics
Proto-Oncogene Proteins c-mdm2 metabolism
Rats
Reactive Oxygen Species metabolism
Superoxide Dismutase genetics
Superoxide Dismutase metabolism
Tumor Suppressor Protein p53 genetics
Antioxidants metabolism
DNA Damage
Oryza metabolism
Plant Proteins metabolism
Tumor Suppressor Protein p53 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1097-0010
- Volume :
- 99
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Journal of the science of food and agriculture
- Publication Type :
- Academic Journal
- Accession number :
- 31250448
- Full Text :
- https://doi.org/10.1002/jsfa.9888