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Further Insights into the Architecture of the P N Promoter That Controls the Expression of the bzd Genes in Azoarcus .
- Source :
-
Genes [Genes (Basel)] 2019 Jun 27; Vol. 10 (7). Date of Electronic Publication: 2019 Jun 27. - Publication Year :
- 2019
-
Abstract
- The anaerobic degradation of benzoate in bacteria involves the benzoyl-CoA central pathway. Azoarcus /Aromatoleum strains are a major group of anaerobic benzoate degraders, and the transcriptional regulation of the bzd genes was extensively studied in Azoarcus sp. CIB. In this work, we show that the bzdR regulatory gene and the P <subscript>N</subscript> promoter can also be identified upstream of the catabolic bzd operon in all benzoate-degrader Azoarcus /Aromatoleum strains whose genome sequences are currently available. All the P <subscript>N</subscript> promoters from Azoarcus /Aromatoleum strains described here show a conserved architecture including three operator regions (ORs), i.e., OR1 to OR3, for binding to the BzdR transcriptional repressor. Here, we demonstrate that, whereas OR1 is sufficient for the BzdR-mediated repression of the P <subscript>N</subscript> promoter, the presence of OR2 and OR3 is required for de-repression promoted by the benzoyl-CoA inducer molecule. Our results reveal that BzdR binds to the P <subscript>N</subscript> promoter in the form of four dimers, two of them binding to OR1. The BzdR/ P <subscript>N</subscript> complex formed induces a DNA loop that wraps around the BzdR dimers and generates a superstructure that was observed by atomic force microscopy. This work provides further insights into the existence of a conserved BzdR-dependent mechanism to control the expression of the bzd genes in Azoarcus strains.<br />Competing Interests: Conflicts of interest: The authors declare that there are no competing interests.
- Subjects :
- Anaerobiosis
Bacterial Proteins chemistry
Benzoates chemistry
Genes, Regulator
Microscopy, Atomic Force
Operator Regions, Genetic genetics
Operon genetics
Operon physiology
Protein Conformation
Transcription Factors genetics
Transcription, Genetic
Acyl Coenzyme A genetics
Azoarcus genetics
Gene Expression Regulation, Bacterial
Promoter Regions, Genetic physiology
Subjects
Details
- Language :
- English
- ISSN :
- 2073-4425
- Volume :
- 10
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Genes
- Publication Type :
- Academic Journal
- Accession number :
- 31252700
- Full Text :
- https://doi.org/10.3390/genes10070489