The effect of diurnal variation in exercise-induced bronchoconstriction.

Objective: Exercise-induced bronchoconstriction (EIB) is an acute, transient narrowing of the airway as a result of exercise. Diurnal variation in asthma is well-established, however, few studies have investigated diurnal variability in EIB; no study has used eucapnic voluntary hyperpnea (EVH). The aim of this study was to examine circadian variability in EIB using EVH. Methods: Fourteen recreationally-active males with mild to moderate asthma and nine healthy controls were randomized to first complete either an AM (07:00-08:00) or PM (17:00-18:00) EVH challenge, followed by the alternate test 34 h to 7 days later. The EVH protocol comprised of six-minutes of hyperventilation of a 5% CO ₂ gas at a minimum ventilation rate of 21 × FEV ₁ min ^-1 . The primary outcome measure was FEV ₁ pre- and post-EVH. Results: We observed no diurnal effect on EIB in the asthma group. The minimum observed post-EVH FEV ₁ in the asthma cohort was 3.58 ± 0.95 L in AM and 3.62 ± 0.87 L in PM tests, corresponding to a 15.0 ± 15.3% vs. 14.9 ± 14.7% reduction from baseline, respectively. The asthma group showed similar baseline FEV ₁ before AM (4.21 ± 0.79 L) and PM (4.25 ± 0.65 L) tests. No difference was observed in minute ventilation between AM (26.1 ± 3.4 × FEV ₁ min ^-1 ) and PM (25.6 ± 3.8 × FEV ₁ min ^-1 ) tests for the asthma cohort. Controls displayed no significant changes in FEV ₁ or minute ventilation between tests. Conclusions: When baseline pulmonary function is similar, this study suggests that time-of-day has no effect on EIB in mild to moderate asthma.