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Long-term imaging of dorsal root ganglia in awake behaving mice.
- Source :
-
Nature communications [Nat Commun] 2019 Jul 12; Vol. 10 (1), pp. 3087. Date of Electronic Publication: 2019 Jul 12. - Publication Year :
- 2019
-
Abstract
- The dorsal root ganglia (DRG) contain the somas of first-order sensory neurons critical for somatosensation. Due to technical difficulties, DRG neuronal activity in awake behaving animals remains unknown. Here, we develop a method for imaging DRG at cellular and subcellular resolution over weeks in awake mice. The method involves the installation of an intervertebral fusion mount to reduce spinal movement, and the implantation of a vertebral glass window without interfering animals' motor and sensory functions. In vivo two-photon calcium imaging shows that DRG neuronal activity is higher in awake than anesthetized animals. Immediately after plantar formalin injection, DRG neuronal activity increases substantially and this activity upsurge correlates with animals' phasic pain behavior. Repeated imaging of DRG over 5 weeks after formalin injection reveals persistent neuronal hyperactivity associated with ongoing pain. The method described here provides an important means for in vivo studies of DRG functions in sensory perception and disorders.
- Subjects :
- Animals
Behavior Observation Techniques instrumentation
Behavior Observation Techniques methods
Behavior, Animal physiology
Calcium chemistry
Female
Formaldehyde administration & dosage
Formaldehyde toxicity
Ganglia, Spinal cytology
Ganglia, Spinal physiology
Green Fluorescent Proteins chemistry
Green Fluorescent Proteins genetics
Intravital Microscopy instrumentation
Male
Mice
Mice, Transgenic
Models, Animal
Optical Imaging instrumentation
Optical Imaging methods
Pain chemically induced
Pain physiopathology
Photons
Wakefulness
Ganglia, Spinal diagnostic imaging
Intravital Microscopy methods
Pain Perception physiology
Sensory Receptor Cells physiology
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 31300648
- Full Text :
- https://doi.org/10.1038/s41467-019-11158-0