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Detection and quantification of 5F-ADB and its methyl ester hydrolysis metabolite in fatal intoxication cases by liquid chromatography-high resolution mass spectrometry.
- Source :
-
Forensic science international [Forensic Sci Int] 2019 Sep; Vol. 302, pp. 109866. Date of Electronic Publication: 2019 Jun 24. - Publication Year :
- 2019
-
Abstract
- 5F-ADB (methyl 2-{[1-(5-fluoropentyl)-1H-indazole-3-carbonyl]amino}-3,3-dimethylbutanoate) is a frequently abused new synthetic cannabinoid that has been sold since at least the end of 2014 on the drug market. It has been classified as an illicit drug in most European countries, and also in Turkey, Japan, and the United States. In this study, 5F-ADB and its methyl ester metabolite were determined in the blood and urine samples taken from fatal cases using liquid chromatography-highresolution mass spectrometry (LC-HRMS). The extraction of samples was performed using a solid-phase extraction method, followed by LC-HRMS analysis. The method was fully validated for linearities, limits of detection (LODs), limits of quantification (LOQs), recoveries, matrix effects, process efficiencies, accuracies, precisions, and stabilities and was applied to 70 blood and 36 urine samples from fatal cases where 5F-ADB was the only drug detected. The LODs were between 0.08 and 0.10ng/mL, and LOQs were between 0.10 and 0.12ng/mL for both blood and urine samples. 5F-ADB and its methyl ester hydrolysis metabolite were found at the blood concentrations ranging from 0.10 to 1.55ng/mL (mean=0.40ng/mL) and 0.15 to 23.4ng/mL (mean=2.69ng/mL), respectively. 5F-ADB was not detected in any urine samples. 5F-ADBmethyl ester hydrolysis metabolite was detected in 35 urine samples with a detection range of 0.28-72.2ng/mL and a mean of 9.02ng/mL. The synthetic cannabinoid 5F-ADB and its methyl ester metabolite were identified and quantified in authentic human blood and/or urine specimens obtained from 70 fatal cases. The method was successfully applied to postmortem blood and urine samples.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Adolescent
Adult
Aged
Cannabinoids poisoning
Chromatography, Liquid
Female
Humans
Hydrolysis
Male
Mass Spectrometry
Methyl Ethers analysis
Middle Aged
Solid Phase Extraction
Substance Abuse Detection methods
Substance-Related Disorders diagnosis
Synthetic Drugs poisoning
Young Adult
Cannabinoids analysis
Forensic Toxicology methods
Synthetic Drugs analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1872-6283
- Volume :
- 302
- Database :
- MEDLINE
- Journal :
- Forensic science international
- Publication Type :
- Academic Journal
- Accession number :
- 31302415
- Full Text :
- https://doi.org/10.1016/j.forsciint.2019.06.024