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Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma.
- Source :
-
Mediators of inflammation [Mediators Inflamm] 2019 Jun 17; Vol. 2019, pp. 7845623. Date of Electronic Publication: 2019 Jun 17 (Print Publication: 2019). - Publication Year :
- 2019
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Abstract
- Objectives: Inhalation of immunostimulatory bacterial DNA segments (cytosine-phosphate-guanosine-oligodeoxynucleotides, CpG-ODN) normalizes clinical and cytologic parameters in severe equine asthma. We hypothesized that CpG-ODN inhalation also reduces the misbalance of elastinolytic activity in asthmatic horses.<br />Methods: Twenty asthmatic horses diagnosed by clinical examinations using a scoring system were included. All horses inhaled CpG-ODNs for 14 days in 2-day intervals. Matrix metalloproteinase (MMP-2/-9) and tissue inhibitors of metalloproteinase (TIMP-1/-2) concentrations were measured in tracheal aspirates using equine sandwich ELISAs before and 2 and 6 weeks after CpG-ODN inhalation.<br />Results: MMP and TIMP concentrations correlated with the results of clinical scoring in all stages of equine asthma. Inhalation therapy led to significant reductions in clinical scores. MMP-2, MMP-9, and TIMP-2 concentrations were significantly reduced immediately, and all MMP and TIMP concentrations 6 weeks after therapy.<br />Discussion: In equine asthma, overexpression of MMPs contributes to pathological tissue destruction, while TIMPs counteract MMPs with overexpression leading to fibrosis formation. The results of this study show that CpG-ODN inhalation may be an effective therapy to address a misbalance in equine asthma.<br />Conclusions: Misbalance of elastinolytic activity seems to improve by CpG-ODN inhalation for at least 6 weeks posttherapy, which may reduce the remodeling of the extracellular matrix. Further studies should evaluate this effect in comparison to glucocorticoid inhalation therapy.<br />Significance: CpG-ODN inhalation may be an effective therapy in the prevention of pulmonary fibrosis formation in equine asthma.
- Subjects :
- Animals
Asthma immunology
Asthma therapy
Extracellular Matrix metabolism
Horse Diseases immunology
Horses
Immunization
Matrix Metalloproteinase 2 metabolism
Matrix Metalloproteinase 9 metabolism
Tissue Inhibitor of Metalloproteinase-1 antagonists & inhibitors
Tissue Inhibitor of Metalloproteinase-1 metabolism
Tissue Inhibitor of Metalloproteinase-2 antagonists & inhibitors
Tissue Inhibitor of Metalloproteinase-2 metabolism
Administration, Inhalation
Asthma veterinary
Horse Diseases therapy
Matrix Metalloproteinase Inhibitors pharmacology
Metalloproteases metabolism
Oligodeoxyribonucleotides administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1466-1861
- Volume :
- 2019
- Database :
- MEDLINE
- Journal :
- Mediators of inflammation
- Publication Type :
- Academic Journal
- Accession number :
- 31316303
- Full Text :
- https://doi.org/10.1155/2019/7845623