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Benzyl butyl phthalate non-linearly affects rat Leydig cell development during puberty.
- Source :
-
Toxicology letters [Toxicol Lett] 2019 Oct 10; Vol. 314, pp. 53-62. Date of Electronic Publication: 2019 Jul 15. - Publication Year :
- 2019
-
Abstract
- Benzyl butyl phthalate (BBP) is a widely used plasticizer and has raised public health concerns. Here, we report the effects of BBP on the testis development during rat puberty. BBP (0, 10, 100 or 1000 mg/kg) was gavaged to 35-day-old male Sprague Dawley rats for 21 days. The serum testosterone levels, Leydig cell number, the expressions of Leydig and Sertoli cell genes and proteins were measured. The in vitro effects on steroidogenesis and gene expression in immature Leydig cells were observed. BBP significantly increased serum testosterone level at 10 mg/kg but lowered its level at 1000 mg/kg without affecting serum luteinizing hormone and follicle-stimulating hormone levels. BBP increased Leydig cell number at all doses but inhibited steroidogenic capacity per Leydig cell at 1000 mg/kg. BBP significantly increased the ratio of phosphos-AKT2 (pAKT2)/AKT2, and phosphos-ERK1/2 (pERK1/2)/ERK1/2 in the testis. Mono-benzyl phthalate (the metabolite of BBP) inhibited steroidogenesis but BBP did not affect androgen production in immature Leydig cells in vitro. In conclusion, BBP non-linearly regulates Leydig cell development by increasing Leydig cell number but inhibiting steroidogenesis.<br /> (Copyright © 2019. Published by Elsevier B.V.)
- Subjects :
- Age Factors
Animals
Dose-Response Relationship, Drug
Extracellular Signal-Regulated MAP Kinases metabolism
Gene Expression Regulation, Developmental
Leydig Cells metabolism
Leydig Cells pathology
Male
Phosphoproteins genetics
Phosphoproteins metabolism
Phosphorylation
Proto-Oncogene Proteins c-akt metabolism
Rats, Sprague-Dawley
Scavenger Receptors, Class B genetics
Scavenger Receptors, Class B metabolism
Signal Transduction drug effects
Testosterone blood
Steroidogenic Acute Regulatory Protein
Cell Proliferation drug effects
Leydig Cells drug effects
Phthalic Acids toxicity
Plasticizers toxicity
Sexual Development drug effects
Testosterone biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3169
- Volume :
- 314
- Database :
- MEDLINE
- Journal :
- Toxicology letters
- Publication Type :
- Academic Journal
- Accession number :
- 31319113
- Full Text :
- https://doi.org/10.1016/j.toxlet.2019.07.016