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A potential probiotic Chromobacterium aquaticum with bacteriocin-like activity enhances the expression of indicator genes associated with nutrient metabolism, growth performance and innate immunity against pathogen infections in zebrafish (Danio rerio).
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2019 Oct; Vol. 93, pp. 124-134. Date of Electronic Publication: 2019 Jul 16. - Publication Year :
- 2019
-
Abstract
- The use of probiotics as alternatives to antibiotics for disease control is a relatively eco-friendly approach in aquaculture; hence, studies isolating and assessing the benefit of potential probiotics to fish farming are common. The zebrafish is an excellent model system for validating beneficial functions of potential probiotics before their practical application in aquaculture. Here, a potentially probiotic Chromobacterium aquaticum was isolated from lake water samples and characterized by biochemical analysis and 16S rDNA sequencing. The probiotic produced extracellular enzymes (protease and xylanase) and a bacteriocin-like substance, which exhibited tolerance to extreme pH and high-temperature conditions and broad-spectrum bactericidal activity against diverse pathogens, including aquatic, foodborne, clinical and plant pathogens. The effects of C. aquaticum on zebrafish nutrient metabolism, growth performance and innate immunity were evaluated by measuring the expression of indicator genes after C. aquaticum feeding for 8 weeks. Fish administered the probiotic exhibited significantly increased hepatic mRNA expression of carbohydrate metabolism-related genes, including glucokinase (GK), hexokinase (HK), glucose-6-phosphatase (G6Pase), and pyruvate kinase (PK-L), and growth-related genes, including the growth hormone receptor (GHR) and insulin-like growth factor-1 (IGF-1). Innate immune-related genes (IL-1β, IL-6, TNF-α, IL-10, IL-21, NF-κb, lysozyme and complement C3b) were induced in fish with probiotic supplementation. Probiotic-treated fish exhibited a higher survival rate than control fish after challenge with Aeromonas hydrophila and Streptococcus iniae. Together, these data suggest that C. aquaticum, as a probiotic feed supplement, could enhance nutrient metabolism and growth performance and could modulate innate immunity against A. hydrophila and S. iniae in zebrafish.<br /> (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Subjects :
- Aeromonas hydrophila physiology
Animal Feed analysis
Animals
Diet veterinary
Dose-Response Relationship, Drug
Gram-Negative Bacterial Infections immunology
Gram-Negative Bacterial Infections veterinary
Random Allocation
Streptococcal Infections immunology
Streptococcal Infections veterinary
Streptococcus iniae physiology
Zebrafish growth & development
Zebrafish metabolism
Bacteriocins pharmacology
Chromobacterium chemistry
Fish Diseases immunology
Gene Expression drug effects
Immunity, Innate drug effects
Probiotics pharmacology
Zebrafish immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 93
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 31323329
- Full Text :
- https://doi.org/10.1016/j.fsi.2019.07.042