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RNA is a critical element for the sizing and the composition of phase-separated RNA-protein condensates.
- Source :
-
Nature communications [Nat Commun] 2019 Jul 19; Vol. 10 (1), pp. 3230. Date of Electronic Publication: 2019 Jul 19. - Publication Year :
- 2019
-
Abstract
- Liquid-liquid phase separation is thought to be a key organizing principle in eukaryotic cells to generate highly concentrated dynamic assemblies, such as the RNP granules. Numerous in vitro approaches have validated this model, yet a missing aspect is to take into consideration the complex molecular mixture and promiscuous interactions found in vivo. Here we report the versatile scaffold ArtiG to generate concentration-dependent RNA-protein condensates within living cells, as a bottom-up approach to study the impact of co-segregated endogenous components on phase separation. We demonstrate that intracellular RNA seeds the nucleation of the condensates, as it provides molecular cues to locally coordinate the formation of endogenous high-order RNP assemblies. Interestingly, the co-segregation of intracellular components ultimately impacts the size of the phase-separated condensates. Thus, RNA arises as an architectural element that can influence the composition and the morphological outcome of the condensate phases in an intracellular context.
- Subjects :
- Cytoplasmic Granules chemistry
HeLa Cells
Humans
Kinetics
Microscopy, Electron, Transmission
Protein Binding
Protein Interaction Maps
RNA chemistry
RNA-Binding Proteins chemistry
Ribonucleoproteins chemistry
Ribonucleoproteins ultrastructure
Cytoplasmic Granules metabolism
RNA metabolism
RNA-Binding Proteins metabolism
Ribonucleoproteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 31324804
- Full Text :
- https://doi.org/10.1038/s41467-019-11241-6