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Dexamethasone action inhibits the release of arachidonic acid from phosphatidylcholine during the suppression of yeast phagocytosis in macrophage cultures.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1988 Jun 16; Vol. 153 (2), pp. 583-90. - Publication Year :
- 1988
-
Abstract
- Dexamethasone suppresses phagocytosis of heat killed Saccharomyces cerevisiae in cultures of murine peritoneal macrophages. Recent observations suggest that dexamethasone induces a phagocytic inhibitory protein that suppresses yeast ingestion by inhibiting macrophage phospholipase A2 activity. The present investigation, therefore, examined whether macrophage lipid metabolism is modulated by dexamethasone. Control and steroid treated macrophages were allowed to incorporate radiolabeled arachidonate and were incubated subsequently in the absence and presence of yeast. Following ingestion by control macrophages, arachidonate from phosphatidylcholine was readily cleaved to free fatty acid and transferred to the neutral lipid fraction. In contrast, arachidonate release was inhibited in dexamethasone treated macrophages. These results suggest that the suppression of yeast phagocytosis by dexamethasone action may be associated with the inhibition of phospholipase A2 activity.
- Subjects :
- Animals
Arachidonic Acid
In Vitro Techniques
Lipid Metabolism
Macrophages drug effects
Mice
Peritoneal Cavity cytology
Phospholipases A2
Saccharomyces cerevisiae
Arachidonic Acids metabolism
Dexamethasone pharmacology
Macrophages physiology
Phagocytosis drug effects
Phosphatidylcholines metabolism
Phospholipases antagonists & inhibitors
Phospholipases A antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 153
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 3132914
- Full Text :
- https://doi.org/10.1016/s0006-291x(88)81135-5