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Otopathogenic Staphylococcus aureus Invades Human Middle Ear Epithelial Cells Primarily through Cholesterol Dependent Pathway.
- Source :
-
Scientific reports [Sci Rep] 2019 Jul 25; Vol. 9 (1), pp. 10777. Date of Electronic Publication: 2019 Jul 25. - Publication Year :
- 2019
-
Abstract
- Chronic suppurative otitis media (CSOM) is one of the most common infectious diseases of the middle ear especially affecting children, leading to delay in language development and communication. Although Staphylococcus aureus is the most common pathogen associated with CSOM, its interaction with middle ear epithelial cells is not well known. In the present study, we observed that otopathogenic S. aureus has the ability to invade human middle ear epithelial cells (HMEECs) in a dose and time dependent manner. Scanning electron microscopy demonstrated time dependent increase in the number of S. aureus on the surface of HMEECs. We observed that otopathogenic S. aureus primarily employs a cholesterol dependent pathway to colonize HMEECs. In agreement with these findings, confocal microscopy showed that S. aureus colocalized with lipid rafts in HMEECs. The results of the present study provide new insights into the pathogenesis of S. aureus induced CSOM. The availability of in vitro cell culture model will pave the way to develop novel effective treatment modalities for CSOM beyond antibiotic therapy.
- Subjects :
- Cells, Cultured
Ear, Middle microbiology
Epithelial Cells metabolism
Epithelial Cells microbiology
Epithelial Cells ultrastructure
Humans
Membrane Microdomains metabolism
Membrane Microdomains microbiology
Otitis microbiology
Staphylococcal Infections microbiology
Staphylococcus aureus pathogenicity
Cholesterol metabolism
Otitis metabolism
Staphylococcal Infections metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 31346200
- Full Text :
- https://doi.org/10.1038/s41598-019-47079-7