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Defects of mitochondrial RNA turnover lead to the accumulation of double-stranded RNA in vivo.
- Source :
-
PLoS genetics [PLoS Genet] 2019 Jul 31; Vol. 15 (7), pp. e1008240. Date of Electronic Publication: 2019 Jul 31 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- The RNA helicase SUV3 and the polynucleotide phosphorylase PNPase are involved in the degradation of mitochondrial mRNAs but their roles in vivo are not fully understood. Additionally, upstream processes, such as transcript maturation, have been linked to some of these factors, suggesting either dual roles or tightly interconnected mechanisms of mitochondrial RNA metabolism. To get a better understanding of the turn-over of mitochondrial RNAs in vivo, we manipulated the mitochondrial mRNA degrading complex in Drosophila melanogaster models and studied the molecular consequences. Additionally, we investigated if and how these factors interact with the mitochondrial poly(A) polymerase, MTPAP, as well as with the mitochondrial mRNA stabilising factor, LRPPRC. Our results demonstrate a tight interdependency of mitochondrial mRNA stability, polyadenylation and the removal of antisense RNA. Furthermore, disruption of degradation, as well as polyadenylation, leads to the accumulation of double-stranded RNAs, and their escape out into the cytoplasm is associated with an altered immune-response in flies. Together our results suggest a highly organised and inter-dependable regulation of mitochondrial RNA metabolism with far reaching consequences on cellular physiology.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Animals
DEAD-box RNA Helicases genetics
DEAD-box RNA Helicases metabolism
DNA-Directed RNA Polymerases genetics
DNA-Directed RNA Polymerases metabolism
Drosophila Proteins genetics
Drosophila melanogaster metabolism
Female
Male
Neoplasm Proteins genetics
Neoplasm Proteins metabolism
Polyadenylation
Polyribonucleotide Nucleotidyltransferase genetics
Polyribonucleotide Nucleotidyltransferase metabolism
RNA Stability
RNA, Antisense chemistry
RNA, Antisense metabolism
RNA, Double-Stranded chemistry
RNA, Double-Stranded metabolism
Drosophila Proteins metabolism
Drosophila melanogaster genetics
RNA, Mitochondrial chemistry
RNA, Mitochondrial metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1553-7404
- Volume :
- 15
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- PLoS genetics
- Publication Type :
- Academic Journal
- Accession number :
- 31365523
- Full Text :
- https://doi.org/10.1371/journal.pgen.1008240