Back to Search
Start Over
Sensitive and Simple Competitive Biomimetic Nanozyme-Linked Immunosorbent Assay for Colorimetric and Surface-Enhanced Raman Scattering Sensing of Triazophos.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2019 Aug 28; Vol. 67 (34), pp. 9658-9666. Date of Electronic Publication: 2019 Aug 16. - Publication Year :
- 2019
-
Abstract
- The biomimetic enzyme-linked immunosorbent assay (BELISA) is widely used for detection of small-molecule compounds as a result of low cost and reagent stability of molecularly imprinted polymers (MIPs). However, enzyme labels used in BELISA still suffer some drawbacks, such as high production cost and limited stability. To overcome the drawbacks, a biomimetic nanozyme-linked immunosorbent assay (BNLISA) based on MIPs and nanozyme labels was first proposed. For nanozyme labels, platinum nanoparticles (PtNPs) acted as peroxidase by catalyzing the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) into an ideal surface-enhanced Raman scattering (SERS) marker. Blue TMB <superscript>2+</superscript> and bovine serum albumin (BSA)-hapten showed superior selectivity when competing with targets for binding sites on MIPs, named the Pt@BSA-hapten probe. The BNLISA method was employed to detect triazophos with a limit of detection of 1 ng mL <superscript>-1</superscript> via colorimetric and SERS methods. Replacing traditional enzymes with nanozymes for combination with MIPs may bring about a new prospect for other compound analyses.
- Subjects :
- Benzidines chemistry
Biomimetic Materials chemistry
Colorimetry instrumentation
Fruit chemistry
Gold chemistry
Immunosorbents chemistry
Metal Nanoparticles chemistry
Platinum chemistry
Pyrus chemistry
Sensitivity and Specificity
Spectrum Analysis, Raman instrumentation
Water Pollutants, Chemical analysis
Colorimetry methods
Organothiophosphates analysis
Pesticides analysis
Spectrum Analysis, Raman methods
Triazoles analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 67
- Issue :
- 34
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 31381330
- Full Text :
- https://doi.org/10.1021/acs.jafc.9b03401