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Knockdown of ANGPTL-4 inhibits inflammatory response and extracellular matrix accumulation in glomerular mesangial cells cultured under high glucose condition.
- Source :
-
Artificial cells, nanomedicine, and biotechnology [Artif Cells Nanomed Biotechnol] 2019 Dec; Vol. 47 (1), pp. 3368-3373. - Publication Year :
- 2019
-
Abstract
- Diabetic nephropathy (DN) is one of the major diabetic complications that lead to end-stage renal failure. Angiopoietin-like protein-4 (ANGPTL-4) has been reported to be dysregulated in diabetes mellitus and diabetic complications. However, the role of ANGPTL-4 in glomerular mesangial cells (MCs) during DN remains unclear. In the present study, we evaluated the role of ANGPTL-4 in MCs in response to high glucose (HG) condition and the potential mechanism. The results proved that ANGPTL-4 expression is significantly increased in HG-stimulated MCs. Knockdown of ANGPTL-4 suppressed HG-induced cell proliferation of MCs. The production of pro-inflammatory cytokines including TNF-α, IL-1β, IL-6 were decreased in ANGPTL-4 knocked down MCs. Inhibition of ANGPTL-4 markedly suppressed the expressions of extracellular matrix (ECM) proteins, collagen IV (Col IV) and fibronectin (FN), in HG-stimulated MCs. Furthermore, ANGPTL-4 knockdown inhibited the HG-induced activation of NF-κB signaling pathway in MCs. Collectively, knockdown of ANGPTL-4 suppressed HG-induced cell proliferation, inflammatory response, and ECM accumulation inhibiting NF-κB signaling pathway in MCs. These findings suggested that ANGPTL-4 might be a therapeutic target for the prevention and treatment of DN.
- Subjects :
- Animals
Cell Line
Cell Proliferation drug effects
Cell Proliferation genetics
Collagen Type IV biosynthesis
Dose-Response Relationship, Drug
Extracellular Matrix drug effects
Fibronectins biosynthesis
Inflammation genetics
Inflammation metabolism
Inflammation pathology
Mesangial Cells pathology
NF-kappa B metabolism
Rats
Signal Transduction drug effects
Signal Transduction genetics
Up-Regulation drug effects
Angiopoietin-Like Protein 4 deficiency
Angiopoietin-Like Protein 4 genetics
Extracellular Matrix metabolism
Gene Knockdown Techniques
Glucose pharmacology
Mesangial Cells drug effects
Mesangial Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2169-141X
- Volume :
- 47
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Artificial cells, nanomedicine, and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 31387395
- Full Text :
- https://doi.org/10.1080/21691401.2019.1649274