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Starch-regulated copper-terephthalic acid as a pH/hydrogen peroxide simultaneous-responsive fluorescent probe for lysosome imaging.
- Source :
-
Dalton transactions (Cambridge, England : 2003) [Dalton Trans] 2019 Sep 14; Vol. 48 (34), pp. 13017-13025. Date of Electronic Publication: 2019 Aug 12. - Publication Year :
- 2019
-
Abstract
- Lysosome visualization is very important for accurate diagnosis of human diseases. However, currently developed lysosome imaging probes usually have poor specificity and are easily quenched, leading to a low signal to noise ratio in lysosome labeling. To resolve this problem, herein, metal-organic framework-based probes of copper-terephthalic acid (CuBDC) are investigated, which show sensitivity to pH and hydrogen peroxide (H <subscript>2</subscript> O <subscript>2</subscript> ), simultaneously. By self-assembling under the template effect of soluble starch, the particle size of CuBDC can be well controlled for entering into cells and locating lysosomes. Based on the Fenton-like reaction, CuBDC can catalyze the decomposition of H <subscript>2</subscript> O <subscript>2</subscript> into ˙OH, which in turn reacts with CuBDC to generate a stable fluorescent substance. Meanwhile, Cu <superscript>2+</superscript> can be released from CuBDC under acidic conditions for reacting with H <subscript>2</subscript> O <subscript>2</subscript> more thoroughly. And the synthesized CuBDC has a similar attraction to the electrophilic ˙OH at different pH values owing to the residual soluble starch in the particles. The above properties cause CuBDC to have a stable fluorescence signal with low pH values and high H <subscript>2</subscript> O <subscript>2</subscript> concentration, simultaneously. The fluorescence imaging experiments in HeLa cells demonstrate that CuBDC acting as a pH/H <subscript>2</subscript> O <subscript>2</subscript> responsive fluorescent probe holds great promise for lysosome-specific imaging.
Details
- Language :
- English
- ISSN :
- 1477-9234
- Volume :
- 48
- Issue :
- 34
- Database :
- MEDLINE
- Journal :
- Dalton transactions (Cambridge, England : 2003)
- Publication Type :
- Academic Journal
- Accession number :
- 31403139
- Full Text :
- https://doi.org/10.1039/c9dt02193k