Two copolymer-grafted silica stationary phases prepared by surface thiol-ene click reaction in deep eutectic solvents for hydrophilic interaction chromatography.

Authors :: Hu Y
Cai T
Zhang H
Chen J
Li Z
Zhao L
Li Z
Qiu H
Source :: Journal of chromatography. A [J Chromatogr A] 2020 Jan 04; Vol. 1609, pp. 460446. Date of Electronic Publication: 2019 Aug 09.
Publication Year :: 2020
Abstract: Two new copolymer-grafted silica stationary phases were prepared and employed in hydrophilic interaction chromatography (HILIC). 2-(Dimethylamino)ethyl methacrylate (DMAEMA) are copolymerized with itaconic acid (IA) and acrylic acid (AA) respectively, via thiol-ene click reaction on silica surface with deep eutectic solvents (DES) as new solvents. The obtained poly(DMAEMA-co-itaconic acid)-grafted silica (Sil-PDM-PIA) and poly(DMAEMA-co-acrylic acid)-grafted silica (Sil-PDM-PAA) were characterized by Fourier transform infrared spectroscopy, elemental analysis and solid-state <superscript>13</superscript> C NMR spectra. Their hydrophilic interaction performances were evaluated by separating nucleosides, nucleobases, saccharides, and amino acids. Compared with previous reported poly(itaconic acid)-grafted silica (Sil-PIA) and poly(acrylic acid)-grafted silica (Sil-PAA) stationary phases, these two new copolymer-grafted silica performed higher selectivity and better separation for polar analytes in HILIC.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)

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