Optimization of enzymatic hydrolysis of cellulosic fraction obtained from stranded driftwood feedstocks for lipid production by Solicoccozyma terricola .

Stranded driftwood feedstocks may represent, after pretreatment with steam explosion and enzymatic hydrolysis, a cheap C-source for producing biochemicals and biofuels using oleaginous yeasts. The hydrolysis was optimized using a response surface methodology (RSM). The solid loading (SL) and the dosage of enzyme cocktail (ED) were variated following a central composite design (CCD) aimed at optimizing the conversion of carbohydrates into lipids (Y _L ) by the yeast Solicoccozyma terricola DBVPG 5870. A second-order polynomial equation was computed for describing the effect of ED and SL on Y _L . The best combination (ED = 3.10%; SL = 22.07%) for releasing the optimal concentration of carbohydrates which gave the highest predicted Y _L (27.32%) was then validated by a new hydrolysis. The resulting value of Y _L (25.26%) was close to the theoretical maximum value. Interestingly, fatty acid profile achieved under the optimized conditions was similar to that reported for palm oil.
Competing Interests: The authors declare that they have no competing interests. On behalf of all co-authors, I confirm that: - There is no conflict of interest. - All authors have approved the manuscript for submission. - The content of the manuscript is original, it has not been published, or submitted for publication elsewhere.