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Optimization of enzymatic hydrolysis of cellulosic fraction obtained from stranded driftwood feedstocks for lipid production by Solicoccozyma terricola .
- Source :
-
Biotechnology reports (Amsterdam, Netherlands) [Biotechnol Rep (Amst)] 2019 Aug 07; Vol. 24, pp. e00367. Date of Electronic Publication: 2019 Aug 07 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Stranded driftwood feedstocks may represent, after pretreatment with steam explosion and enzymatic hydrolysis, a cheap C-source for producing biochemicals and biofuels using oleaginous yeasts. The hydrolysis was optimized using a response surface methodology (RSM). The solid loading (SL) and the dosage of enzyme cocktail (ED) were variated following a central composite design (CCD) aimed at optimizing the conversion of carbohydrates into lipids (Y <subscript>L</subscript> ) by the yeast Solicoccozyma terricola DBVPG 5870. A second-order polynomial equation was computed for describing the effect of ED and SL on Y <subscript>L</subscript> . The best combination (ED = 3.10%; SL = 22.07%) for releasing the optimal concentration of carbohydrates which gave the highest predicted Y <subscript>L</subscript> (27.32%) was then validated by a new hydrolysis. The resulting value of Y <subscript>L</subscript> (25.26%) was close to the theoretical maximum value. Interestingly, fatty acid profile achieved under the optimized conditions was similar to that reported for palm oil.<br />Competing Interests: The authors declare that they have no competing interests. On behalf of all co-authors, I confirm that: - There is no conflict of interest. - All authors have approved the manuscript for submission. - The content of the manuscript is original, it has not been published, or submitted for publication elsewhere.
Details
- Language :
- English
- ISSN :
- 2215-017X
- Volume :
- 24
- Database :
- MEDLINE
- Journal :
- Biotechnology reports (Amsterdam, Netherlands)
- Publication Type :
- Academic Journal
- Accession number :
- 31453116
- Full Text :
- https://doi.org/10.1016/j.btre.2019.e00367