Back to Search Start Over

Nonlinear Regulation of Enzyme-Free DNA Circuitry with Ultrasensitive Switches.

Authors :
Lai W
Xiong X
Wang F
Li Q
Li L
Fan C
Pei H
Source :
ACS synthetic biology [ACS Synth Biol] 2019 Sep 20; Vol. 8 (9), pp. 2106-2112. Date of Electronic Publication: 2019 Sep 04.
Publication Year :
2019

Abstract

DNA is used to construct synthetic chemical reaction networks (CRNs), such as inorganic oscillators and gene regulatory networks. Nonlinear regulation with a simpler molecular mechanism is particularly important in large-scale CRNs with complex dynamics, such as bistability, adaptation, and oscillation of cellular functions. Here we introduce a new approach based on ultrasensitive switches as modular regulatory elements to nonlinearly regulate DNA-based CRNs. The nonlinear behavior of the systems can be finely tuned by programmable regulation of the linker length and the ligand binding sites, of which the Hill coefficients ( n <subscript>H</subscript> ) are in the range of 1.00-2.32. By integrating two different strand displacement reactions with low-order nonlinearities ( n <subscript>H</subscript> ≈ 1.44 and 1.54), we could construct CRNs exhibiting high-order nonlinearities with Hill coefficients of up to ∼2.70. In addition, this could provide an efficient approach for designing CRNs at will with complex chemical dynamics by incorporating our design with previously developed enzyme-free DNA circuits.

Details

Language :
English
ISSN :
2161-5063
Volume :
8
Issue :
9
Database :
MEDLINE
Journal :
ACS synthetic biology
Publication Type :
Academic Journal
Accession number :
31461263
Full Text :
https://doi.org/10.1021/acssynbio.9b00208