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Construction of a novel cell-trappable fluorescent probe for hydrogen sulfide (H 2 S) and its bio-imaging application.
- Source :
-
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2019 Nov; Vol. 411 (27), pp. 7127-7136. Date of Electronic Publication: 2019 Aug 29. - Publication Year :
- 2019
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Abstract
- Fluorescence detection of H <subscript>2</subscript> S in living organisms is greatly advantageous because it is nondestructive and can be used for in situ analysis. We have constructed a novel rhodamine analogue dye (Rho630) by extending the conjugated system of rhodamine to create a novel cell-trappable H <subscript>2</subscript> S fluorescent probe Rho630-AM-H <subscript>2</subscript> S with red light emission. Its application for H <subscript>2</subscript> S fluorescence detection in living HeLa cells and zebrafish was investigated. As expected, Rho630-AM-H <subscript>2</subscript> S showed a huge fluorescence turn-on response of about 20-fold at 630 nm and good selectivity toward H <subscript>2</subscript> S in solution. An MTT assay demonstrated that the probe showed negligible cytotoxicity in the concentrations typically used in fluorescence imaging experiments. Cell imaging experiments revealed that compared with compound 4 without cell-trappable unit modification, Rho630-AM-H <subscript>2</subscript> S exhibited remarkably enhanced cell penetration ability, as an enormous fluorescence signal increase was observed at the red channel within 5 min after Rho630-AM-H <subscript>2</subscript> S was incubated with HeLa cells. Finally, the probe Rho630-AM-H <subscript>2</subscript> S was used to detect H <subscript>2</subscript> S in living HeLa cells and zebrafish with great fluorescence enhancement in the red channel. Graphical abstract.
Details
- Language :
- English
- ISSN :
- 1618-2650
- Volume :
- 411
- Issue :
- 27
- Database :
- MEDLINE
- Journal :
- Analytical and bioanalytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 31468080
- Full Text :
- https://doi.org/10.1007/s00216-019-02090-9