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Novel hyaluronic acid-tyrosine/collagen-based injectable hydrogels as soft filler for tissue engineering.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2019 Dec 01; Vol. 141, pp. 700-712. Date of Electronic Publication: 2019 Aug 29. - Publication Year :
- 2019
-
Abstract
- In this study, we fabricated multifunctionalized hydrogel scaffolds based on hyaluronic acid (HA)-tyrosine and human-like collagen (HLC) by crosslinking with 1, 4-butanedioldiglycidyl ether (BDDE) for soft tissue fillers. The physicochemical of HA-tyrosine/BDDE (HTB) and various proportions of HA-tyrosine/BDDE/HLC (HTBH) hydrogels were characterized by swelling ratio, mechanical strength, morphology and thermal stability. The results demonstrated various HTBH hydrogels had superior performance in mechanical properties than HTB hydrogels. The biodegradation in vitro results demonstrated the degradation of HTBH hydrogels were slower than HTB hydrogels, and residual masses of HTBH hydrogels varied with different proportions of HA-tyrosine and HLC in the presence of the combination of HAse/collagenase I. The cell counting kit-8 (CCK-8), Hoechst/PI staining and cell adhesion of various HTBH hydrogels showed less cytotoxic and superior fibroblast attachment than those of HTB hydrogels. Moreover, subcutaneous injections of HTBH hydrogels containing high proportions of HLC in mice and rabbits all exhibit better biocompatibility and anti-biodegradation compared to HTB hydrogels after 1, 4, 8, 12 and 16 weeks. Hematoxylin-eosin staining (H&E), immunohistochemical and transmission electron microscope (TEM) analysis results indicated HTBH hydrogels had less intense inflammatory responses with increase proportion of HLC. Taken together, HTBH hydrogels had great potential application as ideal soft tissue fillers with excellent mechanical properties, biological stability and biocompatibility.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Biocompatible Materials administration & dosage
Biocompatible Materials chemistry
Biocompatible Materials pharmacology
Cell Adhesion drug effects
Cell Line
Hydrogels administration & dosage
Injections
Male
Materials Testing
Mice
Porosity
Rabbits
Collagen chemistry
Hyaluronic Acid chemistry
Hydrogels chemistry
Hydrogels pharmacology
Tissue Engineering
Tyrosine chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 141
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 31473315
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2019.08.233