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Early-Life Compartmentalization of Immune Cells in Human Fetal Tissues Revealed by High-Dimensional Mass Cytometry.
- Source :
-
Frontiers in immunology [Front Immunol] 2019 Aug 14; Vol. 10, pp. 1932. Date of Electronic Publication: 2019 Aug 14 (Print Publication: 2019). - Publication Year :
- 2019
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Abstract
- The human fetal immune system must protect the infant against the sudden exposure to a large variety of pathogens upon birth. While it is known that the fetal immune system develops in sequential waves, relatively little is known about the composition of the innate and adaptive immune system in the tissues. Here, we applied high-dimensional mass cytometry to profile the immune system in human fetal liver, spleen, and intestine. With Hierarchical Stochastic Neighbor Embedding (HSNE) we distinguished 177 distinct immune cell clusters, including both previously identified and novel cell clusters. PCA analysis indicated substantial differences between the compositions of the immune system in the different organs. Through dual t-SNE we identified tissue-specific cell clusters, which were found both in the innate and adaptive compartment. To determine the spatial location of tissue-specific subsets we developed a 31-antibody panel to reveal both the immune compartment and surrounding stromal elements through analysis of snap-frozen tissue samples with imaging mass cytometry. Imaging mass cytometry reconstructed the tissue architecture and allowed both the characterization and determination of the location of the various immune cell clusters within the tissue context. Moreover, it further underpinned the distinctness of the immune system in the tissues. Thus, our results provide evidence for early compartmentalization of the adaptive and innate immune compartment in fetal spleen, liver, and intestine. Together, our data provide a unique and comprehensive overview of the composition and organization of the human fetal immune system in several tissues.
- Subjects :
- Adaptive Immunity immunology
Cell Lineage immunology
Cluster Analysis
Fetus cytology
Humans
Immune System cytology
Immune System embryology
Immunity, Innate immunology
Intestines cytology
Intestines embryology
Intestines immunology
Liver cytology
Liver embryology
Liver immunology
Principal Component Analysis
Spleen cytology
Spleen embryology
Spleen immunology
T-Lymphocytes classification
T-Lymphocytes immunology
Fetus immunology
Flow Cytometry methods
Immune System immunology
Single-Cell Analysis methods
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 10
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 31474997
- Full Text :
- https://doi.org/10.3389/fimmu.2019.01932