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Urine and Free Immunoglobulin Light Chains as Analytes for Serodiagnosis of Hantavirus Infection.
- Source :
-
Viruses [Viruses] 2019 Sep 01; Vol. 11 (9). Date of Electronic Publication: 2019 Sep 01. - Publication Year :
- 2019
-
Abstract
- Rapid point-of-care testing is a megatrend in infectious disease diagnosis. We have introduced a homogeneous immunoassay concept, which is based on the simultaneous binding of antigen and protein L to a given immunoglobulin molecule. The complex formation is detected utilizing time-resolved Förster resonance energy transfer between antigen-attached donor and acceptor-labeled protein L, hence the name LFRET. Here, we demonstrate that urine can be used as a sample matrix in LFRET-based serodiagnostics. We studied urine samples collected during the hospitalization and recovery of patients with acute Puumala orthohantavirus (PUUV) infection. We compared PUUV antibody-specific LFRET signals in urine to those in plasma, and found excellent correlation in the test outcomes The LFRET test from urine was positive in 40/40 patients with acute PUUV infection. PUUV causes a mild form of hemorrhagic fever with renal syndrome, characterized by acute kidney injury and proteinuria. Immunofluorescence and western blotting demonstrated PUUV-IgG and -IgA in urine, however, the presence of intact immunoglobulins did not fully explain the LFRET signals. We purified free light chains (FLCs) from both urine and serum of healthy volunteers and patients with acute PUUV infection, and verified the presence of antigen-specific FLCs. Antigen-specific FLCs provide a new means for non-invasive antibody detection and disease diagnosis.<br />Competing Interests: The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. Some of the authors are listed as inventors in a patent (EP3111218B1, https://patents.google.com/patent/EP3111218B1/en) owned by University of Helsinki.
- Subjects :
- Antibodies, Viral urine
Capsid Proteins immunology
Orthohantavirus immunology
Hantavirus Infections urine
Hemorrhagic Fever with Renal Syndrome diagnosis
Hemorrhagic Fever with Renal Syndrome urine
Humans
Immunoassay
Immunoglobulin A urine
Immunoglobulin G urine
Immunoglobulin Light Chains immunology
Point-of-Care Testing
Puumala virus immunology
Puumala virus isolation & purification
Viral Core Proteins immunology
Orthohantavirus isolation & purification
Hantavirus Infections diagnosis
Immunoglobulin Light Chains urine
Serologic Tests methods
Subjects
Details
- Language :
- English
- ISSN :
- 1999-4915
- Volume :
- 11
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Viruses
- Publication Type :
- Academic Journal
- Accession number :
- 31480594
- Full Text :
- https://doi.org/10.3390/v11090809