Volume-CLEM: a method for correlative light and electron microscopy in three dimensions.

Authors :: Hegermann J
Wrede C
Fassbender S
Schliep R
Ochs M
Knudsen L
Mühlfeld C
Source :: American journal of physiology. Lung cellular and molecular physiology [Am J Physiol Lung Cell Mol Physiol] 2019 Dec 01; Vol. 317 (6), pp. L778-L784. Date of Electronic Publication: 2019 Sep 11.
Publication Year :: 2019
Abstract: Generation of three-dimensional (3D) data sets from serial sections of tissues imaged by light microscopy (LM) allows identification of rare structures by morphology or fluorescent labeling. Here, we demonstrate a workflow for correlative LM and electron microscopy (EM) from 3D LM to 3D EM, using the same sectioned material for both methods consecutively. The new approach is easy to reproduce in routine EM laboratories and applicable to a wide range of organs and research questions.

Subjects :: Animals
Mice
Mice, Inbred C57BL
Imaging, Three-Dimensional methods
Lung ultrastructure
Microscopy, Electron, Scanning methods
Microscopy, Fluorescence methods

Language :: English
ISSN :: 1522-1504
Volume :: 317
Issue :: 6
Database :: MEDLINE
Journal :: American journal of physiology. Lung cellular and molecular physiology
Publication Type :: Academic Journal
Accession number :: 31508976
Full Text :: https://doi.org/10.1152/ajplung.00333.2019

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