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Three-Dimensional Paper-Based Microfluidic Analytical Devices Integrated with a Plasma Separation Membrane for the Detection of Biomarkers in Whole Blood.
- Source :
-
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2019 Oct 09; Vol. 11 (40), pp. 36428-36434. Date of Electronic Publication: 2019 Sep 25. - Publication Year :
- 2019
-
Abstract
- Paper-based microfluidic analytical devices (μPADs) have recently attracted attention as a point-of-care test kit because of their low cost and nonrequirement for external forces. To directly detect biomarkers in whole blood, however, they need to be assembled with a filter such as a plasma separation membrane (PSM) because the color of the blood cells interferes with the colorimetric assay. However, this assembly process is rather complicated and cumbersome, and the fluid does not uniformly move to the detection zone when the adhesion between the paper and PSM is not perfect. In this study, we report a simple three-dimensional (3D) printing method for fabricating PSM-integrated 3D-μPADs made of plastics without the need for additional assembly. In detail, PSM was coated with parylene C to prevent its dissolution from organic solvent during 3D printing. Then, the coated PSM was superimposed on the paper. Detection zones and a reservoir were printed on the paper and PSM via liquid photopolymerization, using a digital light processing printer. The limit of detection of the PSM-integrated 3D-μPADs for glucose in whole blood was 0.3 mM, and these devices demonstrated clinically relevant performance on diabetes patient blood samples. Our 3D-μPADs can also simultaneously detect multiple metabolic disease markers including glucose, cholesterol, and triglycerides in whole blood. Our results suggest that our printing method is useful for fabricating 3D-μPADs integrated with PSM for the direct detection of biomarkers in whole blood.
- Subjects :
- Blood Glucose analysis
Cholesterol blood
Coated Materials, Biocompatible chemistry
Diabetes Mellitus blood
Humans
Polymers chemistry
Printing, Three-Dimensional
Triglycerides blood
Ultraviolet Rays
Xylenes chemistry
Biomarkers blood
Lab-On-A-Chip Devices
Membranes, Artificial
Paper
Plasma chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1944-8252
- Volume :
- 11
- Issue :
- 40
- Database :
- MEDLINE
- Journal :
- ACS applied materials & interfaces
- Publication Type :
- Academic Journal
- Accession number :
- 31512861
- Full Text :
- https://doi.org/10.1021/acsami.9b13644