Back to Search
Start Over
Flavobacterium cellulosilyticum sp. nov., a novel psychrophilic bacterium isolated from Arctic soil.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2020 Jan; Vol. 70 (1), pp. 44-50. - Publication Year :
- 2020
-
Abstract
- A novel psychrophilic, light-yellow-coloured, aerobic, Gram-stain-negative, rod-shaped, non-motile bacterium, designated strain AR-3-4 <superscript>T</superscript> was isolated from a sample of Arctic soil. Strain AR-3-4 <superscript>T</superscript> grew at 0-25 °C, pH 6.0-9.0 and 0-1.0 % (w/v) NaCl concentration. The 16S rRNA gene sequence analysis showed that strain AR-3-4 <superscript>T</superscript> belonged to the genus Flavobacterium , with nearest phylogenetic neighbour being Flavobacterium fluvii H7 <superscript>T</superscript> (97.5 % sequence similarity). The strain comprised phosphatidylethanolamine as the main polar lipid, MK-6 as predominant respiratory quinone, and summed feature 3 (C <subscript>16 : 1</subscript> ω7 c and/or C <subscript>16 : 1</subscript> ω6 c ), anteiso-C <subscript>15 : 0</subscript> and iso-C <subscript>15 : 0</subscript> as the major fatty acids. The average nucleotide identity and in silico DNA-DNA hybridization values between strain AR-3-4 <superscript>T</superscript> and closest members were below the threshold values of 95 % and 70 %, respectively. The DNA G+C content was 33.0 mol%. Based on the polyphasic taxonomic data, the novel species Flavobacterium cellulosilyticum sp. nov. is proposed with the type strain AR-3-4 <superscript>T</superscript> (=KEMB 9005-740 <superscript>T</superscript> =KACC 21171 <superscript>T</superscript> =NBRC 113787 <superscript>T</superscript> ).
- Subjects :
- Arctic Regions
Bacterial Typing Techniques
Base Composition
DNA, Bacterial genetics
Fatty Acids chemistry
Flavobacterium isolation & purification
Nucleic Acid Hybridization
Phosphatidylethanolamines chemistry
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Vitamin K 2 analogs & derivatives
Vitamin K 2 chemistry
Flavobacterium classification
Phylogeny
Soil Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 70
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 31513007
- Full Text :
- https://doi.org/10.1099/ijsem.0.003707