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Long-term use of interferon-β in multiple sclerosis increases Vδ1 - Vδ2 - Vγ9 - γδ T cells that are associated with a better outcome.
- Source :
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Journal of neuroinflammation [J Neuroinflammation] 2019 Sep 13; Vol. 16 (1), pp. 179. Date of Electronic Publication: 2019 Sep 13. - Publication Year :
- 2019
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Abstract
- Background: We previously reported that Vδ2 <superscript>+</superscript> Vγ9 <superscript>+</superscript> γδ T cells were significantly decreased in multiple sclerosis (MS) patients without disease-modifying therapies (untreated MS) and were negatively correlated with Expanded Disability Status Scale (EDSS) scores, suggesting protective roles of Vδ2 <superscript>+</superscript> Vγ9 <superscript>+</superscript> γδ T cells. Interferon-β (IFN-β) is one of the first-line disease-modifying drugs for MS. However, no previous studies have reported changes in γδ T cell subsets under IFN-β treatment. Therefore, we aimed to clarify the effects of the long-term usage of IFN-β on γδ T cell subsets in MS patients.<br />Methods: Comprehensive flow cytometric immunophenotyping was performed in 35 untreated MS and 21 MS patients on IFN-β for more than 2 years (IFN-β-treated MS) including eight super-responders fulfilling no evidence of disease activity criteria, and 44 healthy controls (HCs).<br />Results: The percentages of Vδ2 <superscript>+</superscript> Vγ9 <superscript>+</superscript> cells in γδ T cells were significantly lower in untreated and IFN-β-treated MS patients than in HCs. By contrast, the percentages of Vδ1 <superscript>-</superscript> Vδ2 <superscript>-</superscript> Vγ9 <superscript>-</superscript> cells in γδ T cells were markedly higher in IFN-β-treated MS patients than in HCs and untreated MS patients (both p < 0.001). A significant negative correlation between the percentages of Vδ2 <superscript>+</superscript> Vγ9 <superscript>+</superscript> cells in γδ T cells and EDSS scores was confirmed in untreated MS but not evident in IFN-β-treated MS. Moreover, class-switched memory B cells were decreased in IFN-β-treated MS compared with HCs (p < 0.001) and untreated MS patients (p = 0.006). Interestingly, the percentages of Vδ1 <superscript>-</superscript> Vδ2 <superscript>-</superscript> Vγ9 <superscript>-</superscript> cells in γδ T cells were negatively correlated with class-switched memory B cell percentages in all MS patients (r = - 0.369, p = 0.005), and the percentages of Vδ1 <superscript>-</superscript> Vδ2 <superscript>-</superscript> Vγ9 <superscript>-</superscript> cells in Vδ1 <superscript>-</superscript> Vδ2 <superscript>-</superscript> γδ T cells were negatively correlated with EDSS scores only in IFN-β super-responders (r = - 0.976, p < 0.001).<br />Conclusions: The present study suggests that long-term usage of IFN-β increases Vδ1 <superscript>-</superscript> Vδ2 <superscript>-</superscript> Vγ9 <superscript>-</superscript> γδ T cells, which are associated with a better outcome, especially in IFN-β super-responders. Thus, increased Vδ1 <superscript>-</superscript> Vδ2 <superscript>-</superscript> Vγ9 <superscript>-</superscript> cells together with decreased class-switched memory B cells may contribute to the suppression of disease activity in MS patients under IFN-β treatment.
- Subjects :
- Adult
Female
Humans
Male
Middle Aged
Receptors, Antigen, T-Cell, gamma-delta
T-Lymphocyte Subsets immunology
Immunologic Factors therapeutic use
Interferon-beta therapeutic use
Multiple Sclerosis, Relapsing-Remitting drug therapy
Multiple Sclerosis, Relapsing-Remitting immunology
T-Lymphocyte Subsets drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1742-2094
- Volume :
- 16
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of neuroinflammation
- Publication Type :
- Academic Journal
- Accession number :
- 31519178
- Full Text :
- https://doi.org/10.1186/s12974-019-1574-5