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Type 2 diabetes differentially affects the substrate saturation kinetic attributes of erythrocyte hexokinase and phosphofructokinase.
- Source :
-
FEBS letters [FEBS Lett] 2020 Jan; Vol. 594 (2), pp. 240-250. Date of Electronic Publication: 2019 Sep 25. - Publication Year :
- 2020
-
Abstract
- The substrate kinetic parameters of hexokinase (HK) and phosphofructokinase (PFK)-the key irreversible enzymes of glycolysis-in erythrocytes from type 2 diabetic subjects were examined in comparison with control subjects. It was observed that the kinetic parameters such as K <subscript>m</subscript> , V <subscript>max</subscript> , Apparent K <subscript>cat</subscript> , K <subscript>cat</subscript> /K <subscript>m</subscript> , and substrate (ATP) inhibition kinetic and substrate binding characteristics are significantly altered in the diabetic group. The observed changes are suggestive of compositional changes in the subunit makeup of HK and PFK. The implication of these findings in relation to energy status of the diabetic erythrocyte and its interrelationship with loss of cell deformability are discussed here.<br /> (© 2019 Federation of European Biochemical Societies.)
- Subjects :
- Adenosine Triphosphate chemistry
Adult
Diabetes Mellitus, Type 2 drug therapy
Diabetes Mellitus, Type 2 enzymology
Diabetes Mellitus, Type 2 pathology
Erythrocytes enzymology
Female
Glycolysis genetics
Hexokinase chemistry
Humans
Hypoglycemic Agents
Kinetics
Male
Middle Aged
Phosphofructokinases chemistry
Protein Binding genetics
Substrate Specificity
Diabetes Mellitus, Type 2 blood
Hexokinase blood
Phosphofructokinases blood
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3468
- Volume :
- 594
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 31520487
- Full Text :
- https://doi.org/10.1002/1873-3468.13604