Anti-Inflammatory and anti-proliferative oleanane-type triterpene glycosides from the vine of Momordica cochinchinensis .

This research isolated two new oleanane-type triterpene glycosides, named mocochinosides A ( 1 ) and B ( 2 ), together with ten known compounds as chikusetsusaponin IVa ethyl ester ( 3 ), momordin Ib ( 4) , momordin IIb ( 5 ), momordin II ( 6 ), calenduloside G ( 7 ), calenduloside H ( 8 ), elatoside A ( 9 ), elatoside C ( 10 ), calendulaglycoside C 6'- O -7-butyl ester ( 11 ), and hederagenin 3- O - β -D-glucuronopyranoside ( 12 ) and characterized them from the vines of Momordica cochinchinensis . The new structures of both glycosides 1 - 2 were elucidated by spectroscopic analysis including 2 D NMR and MS, followed by an analysis of their anti-inflammatory and cytotoxic activities. Compounds 1 , 4 , and 11 showed moderate inhibitions for NO production on RAW264.7 macrophages induced by LPS at IC ₅₀ 5.41 ∼ 11.28 μM. Compounds 3 , 4 , and 7 (IC ₅₀ 8.42 ∼ 19.74 μM) exhibited potential anti-proliferative activities against both of WiDr and MCF-7 human tumor cell lines.