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Polyinosinic-polycytidylic acid accelerates intestinal stem cell proliferation via modulating Myc expression.
- Source :
-
Journal of cellular physiology [J Cell Physiol] 2020 Apr; Vol. 235 (4), pp. 3646-3656. Date of Electronic Publication: 2019 Sep 26. - Publication Year :
- 2020
-
Abstract
- It is well known that exposure of double-stranded RNA (dsRNA) to intestine immediately induces villus damage with severe diarrhea, which is mediated by toll-like receptor 3 signaling activation. However, the role of intestinal stem cells (ISCs) remains obscure during the pathology. In the present study, polyinosinic-polycytidylic acid (poly[I:C]), mimicking viral dsRNA, was used to establish intestinal damage model. Mice were acutely and chronically exposed to poly(I:C), and ISCs in jejunum were analyzed. The results showed that the height of villus was shorter 48 hr after acute poly(I:C) exposure compared with that of controls, while chronic poly(I:C) treatment increased both villus height and crypt depth in jejunum compared with control animals. The numbers of ISCs in jejunum were significantly increased after acute and chronic poly(I:C) exposure. Poly (I:C)-stimulated ISCs have stronger capacities to differentiate into intestine endocrine cells. Mechanistically, poly(I:C) treatment increased expression of Stat1 and Axin2 in the intestinal crypt, which was along with increased expression of Myc, Bcl2, and ISC proliferation. These findings suggest that dsRNA exposure could induce ISC proliferation to ameliorate dsRNA-induced intestinal injury.<br /> (© 2019 Wiley Periodicals, Inc.)
- Subjects :
- Animals
Apoptosis drug effects
Axin Protein genetics
Cell Proliferation drug effects
Gene Expression Regulation drug effects
Intestinal Mucosa drug effects
Intestinal Mucosa metabolism
Jejunum drug effects
Jejunum growth & development
Mice
RNA, Double-Stranded drug effects
STAT1 Transcription Factor genetics
Signal Transduction
Toll-Like Receptor 3 genetics
Intestinal Mucosa growth & development
Poly I-C pharmacology
Proto-Oncogene Proteins c-myc genetics
Stem Cells drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4652
- Volume :
- 235
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of cellular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 31559639
- Full Text :
- https://doi.org/10.1002/jcp.29254