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Polyinosinic-polycytidylic acid accelerates intestinal stem cell proliferation via modulating Myc expression.

Authors :
Zeng H
Tang J
Yue M
Cheng J
Fan Y
Li M
Zhang X
Li H
Duan H
Zhang M
Fan G
Zhu Q
Shao L
Source :
Journal of cellular physiology [J Cell Physiol] 2020 Apr; Vol. 235 (4), pp. 3646-3656. Date of Electronic Publication: 2019 Sep 26.
Publication Year :
2020

Abstract

It is well known that exposure of double-stranded RNA (dsRNA) to intestine immediately induces villus damage with severe diarrhea, which is mediated by toll-like receptor 3 signaling activation. However, the role of intestinal stem cells (ISCs) remains obscure during the pathology. In the present study, polyinosinic-polycytidylic acid (poly[I:C]), mimicking viral dsRNA, was used to establish intestinal damage model. Mice were acutely and chronically exposed to poly(I:C), and ISCs in jejunum were analyzed. The results showed that the height of villus was shorter 48 hr after acute poly(I:C) exposure compared with that of controls, while chronic poly(I:C) treatment increased both villus height and crypt depth in jejunum compared with control animals. The numbers of ISCs in jejunum were significantly increased after acute and chronic poly(I:C) exposure. Poly (I:C)-stimulated ISCs have stronger capacities to differentiate into intestine endocrine cells. Mechanistically, poly(I:C) treatment increased expression of Stat1 and Axin2 in the intestinal crypt, which was along with increased expression of Myc, Bcl2, and ISC proliferation. These findings suggest that dsRNA exposure could induce ISC proliferation to ameliorate dsRNA-induced intestinal injury.<br /> (© 2019 Wiley Periodicals, Inc.)

Details

Language :
English
ISSN :
1097-4652
Volume :
235
Issue :
4
Database :
MEDLINE
Journal :
Journal of cellular physiology
Publication Type :
Academic Journal
Accession number :
31559639
Full Text :
https://doi.org/10.1002/jcp.29254