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Conjugative Assembly Genome Engineering (CAGE).

Authors :
Güell M
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2075, pp. 399-409.
Publication Year :
2020

Abstract

Conjugative assembly genome engineering (CAGE) enables the transfer of large chromosomal regions from a donor to a recipient. Specific regions of the donor chromosome can be introduced in the recipient genome by the directed insertion of an origin of transfer and two selection cassettes. Multiple paired CAGE experiments can be combined to generate chimeric chromosomes from different donor strains.

Subjects

Subjects :
Alleles
Bacteria genetics
DNA, Bacterial
Genetic Markers
Genetic Testing
Genomics methods
Genotyping Techniques
Plasmids genetics
Recombination, Genetic
Whole Genome Sequencing
Conjugation, Genetic
Gene Transfer, Horizontal
Genetic Engineering
Genome, Bacterial

Details

Language :
English
ISSN :
1940-6029
Volume :
2075
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
31584178
Full Text :
https://doi.org/10.1007/978-1-4939-9877-7_28
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