Back to Search
Start Over
Basal Sodium-Dependent Vitamin C Transporter 2 polarization in choroid plexus explant cells in normal or scorbutic conditions.
- Source :
-
Scientific reports [Sci Rep] 2019 Oct 08; Vol. 9 (1), pp. 14422. Date of Electronic Publication: 2019 Oct 08. - Publication Year :
- 2019
-
Abstract
- Vitamin C is incorporated into the cerebrospinal fluid (CSF) through choroid plexus cells. While the transfer of vitamin C from the blood to the brain has been studied functionally, the vitamin C transporter, SVCT2, has not been detected in the basolateral membrane of choroid plexus cells. Furthermore, it is unknown how its expression is induced in the developing brain and modulated in scurvy conditions. We concluded that SVCT2 is intensely expressed in the second half of embryonic brain development and postnatal stages. In postnatal and adult brain, SVCT2 is highly expressed in all choroidal plexus epithelial cells, shown by colocalization with GLUT1 in the basolateral membranes and without MCT1 colocalization, which is expressed in the apical membrane. We confirmed that choroid plexus explant cells (in vitro) form a sealed epithelial structure, which polarized basolaterally, endogenous or overexpressed SVCT2. These results are reproduced in vivo by injecting hSVCT2wt-EYFP lentivirus into the CSF. Overexpressed SVCT2 incorporates AA (intraperitoneally injected) from the blood to the CSF. Finally, we observed in Guinea pig brain under scorbutic condition, that normal distribution of SVCT2 in choroid plexus may be regulated by peripheral concentrations of vitamin C. Additionally, we observed that SVCT2 polarization also depends on the metabolic stage of the choroid plexus cells.
- Subjects :
- Animals
Blood-Brain Barrier growth & development
Blood-Brain Barrier metabolism
Brain growth & development
Cell Membrane metabolism
Cells, Cultured
Choroid Plexus metabolism
Embryonic Development genetics
Epithelial Cells metabolism
Epithelial Cells pathology
Gene Expression Regulation, Developmental genetics
Guinea Pigs
Mice
Monocarboxylic Acid Transporters genetics
Neurons metabolism
Sodium-Coupled Vitamin C Transporters cerebrospinal fluid
Swine
Symporters genetics
Ascorbic Acid metabolism
Brain metabolism
Glucose Transporter Type 1 blood
Sodium-Coupled Vitamin C Transporters blood
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 31594969
- Full Text :
- https://doi.org/10.1038/s41598-019-50772-2