Back to Search
Start Over
A Polysaccharide Isolated from Codonopsis pilosula with Immunomodulation Effects Both In Vitro and In Vivo.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2019 Oct 09; Vol. 24 (20). Date of Electronic Publication: 2019 Oct 09. - Publication Year :
- 2019
-
Abstract
- In this study, an acidic polysaccharide from Codonopsis pilosula Nannf. var. modesta (Nannf.) L. T. Shen (WCP-I) and its main fragment, WCP-Ia, obtained after pectinase digestion, were structurally elucidated and found to consist of a rhamnogalacturonan I (RG-I) region containing both arabinogalactan type I (AG-I) and type II (AG-II) as sidechains. They both expressed immunomodulating activity against Peyer's patch cells. Endo-1,4-β-galactanase degradation gave a decrease of interleukine 6 (IL-6) production compared with native WCP-I and WCP-Ia, but exo-α-l-arabinofuranosidase digestion showed no changes in activity. This demonstrated that the stimulation activity partly disappeared with removal of β-d-(1→4)-galactan chains, proving that the AG-I side chain plays an important role in immunoregulation activity. WCP-Ia had a better promotion effect than WCP-I in vivo, shown through an increased spleen index, higher concentrations of IL-6, transforming growth factor-β (TGF-β), and tumor necrosis factor-α (TNF-α) in serum, and a slight increment in the secretory immunoglobulin A (sIgA) and CD4 <superscript>+</superscript> /CD8 <superscript>+</superscript> T lymphocyte ratio. These results suggest that β-d-(1→4)-galactan-containing chains in WCP-I play an essential role in the expression of immunomodulating activity. Combining all the results in this and previous studies, the intestinal immune system might be the target site of WCP-Ia.
- Subjects :
- Animals
Cell Survival drug effects
Hydrolysis
Immunity, Mucosal drug effects
Immunologic Factors chemistry
Mice
Molecular Structure
Monosaccharides chemistry
Peyer's Patches drug effects
Peyer's Patches immunology
Peyer's Patches metabolism
Plant Extracts chemistry
Polysaccharides chemistry
Spectrum Analysis
Codonopsis chemistry
Immunologic Factors pharmacology
Immunomodulation drug effects
Plant Extracts pharmacology
Polysaccharides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 24
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 31600890
- Full Text :
- https://doi.org/10.3390/molecules24203632