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Deficiency of calpain-6 inhibits primary ciliogenesis.
- Source :
-
BMB reports [BMB Rep] 2019 Oct; Vol. 52 (10), pp. 619-624. - Publication Year :
- 2019
-
Abstract
- The primary cilium is a microtubule-based structure projecting from a cell. Although the primary cilium shows no motility, it can recognize environmental stimuli. Thus, ciliary defects cause severe abnormalities called ciliopathies. Ciliogenesis is a very complex process and involves a myriad of components and regulators. In order to excavate the novel positive regulators of ciliogenesis, we performed mRNA microarray using starved NIH/3T3 cells. We selected 62 murine genes with corresponding human orthologs, with significantly upregulated expression at 24 h after serum withdrawal. Finally, calpain-6 was selected as a positive regulator of ciliogenesis. We found that calpain-6 deficiency reduced the percentage of ciliated cells and impaired sonic hedgehog signaling. It has been speculated that this defect might be associated with decreased levels of α-tubulin acetylation at lysine 40. This is the first study to report a novel role of calpain-6 in the formation of primary cilia. [BMB Reports 2019; 52(10): 619-624].
Details
- Language :
- English
- ISSN :
- 1976-670X
- Volume :
- 52
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- BMB reports
- Publication Type :
- Academic Journal
- Accession number :
- 31619317