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The Expression Pattern of p32 in Sheep Muscle and Its Role in Differentiation, Cell Proliferation, and Apoptosis of Myoblasts.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2019 Oct 18; Vol. 20 (20). Date of Electronic Publication: 2019 Oct 18. - Publication Year :
- 2019
-
Abstract
- The complement 1q binding protein C ( C1QBP ), also known as p32 , is highly expressed in rapidly growing tissues and plays a crucial role in cell proliferation and apoptosis. However, there are no data interpreting its mechanisms in muscle development. To investigate the role of p32 in sheep muscle development, an 856 bp cDNA fragment of p32 containing an 837 bp coding sequence that encodes 278 amino acids was analyzed. We then revealed that the expression of p32 in the longissimus and quadricep muscles of fetal sheep was more significantly up-regulated than expression at other developmental stages. Furthermore, we found that the expression of p32 was increased during myoblasts differentiation in vitro. Additionally, the knockdown of p32 in sheep myoblasts effectively inhibited myoblast differentiation, proliferation, and promoted cell apoptosis in vitro. The interference of p32 also changed the energy metabolism from Oxidative Phosphorylation (OXPHOS) to glycolysis and activated AMP-activated protein kinase ( AMPK ) phosphorylation in sheep myoblasts in vitro. Taken together, our data suggest that p32 plays a vital role in the development of sheep muscle and provides a potential direction for future research on muscle development and some muscle diseases.
- Subjects :
- AMP-Activated Protein Kinases metabolism
Amino Acid Sequence
Animals
Base Sequence
Cell Proliferation
Cloning, Molecular
Energy Metabolism
Glycolysis
Phosphorylation
Sequence Analysis, DNA
Sheep
Apoptosis genetics
Cell Differentiation genetics
Gene Expression Regulation
Mitochondrial Proteins genetics
Muscle, Skeletal metabolism
Myoblasts cytology
Myoblasts metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 20
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 31635221
- Full Text :
- https://doi.org/10.3390/ijms20205161