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Tel1 Activation by the MRX Complex Is Sufficient for Telomere Length Regulation but Not for the DNA Damage Response in Saccharomyces cerevisiae .
- Source :
-
Genetics [Genetics] 2019 Dec; Vol. 213 (4), pp. 1271-1288. Date of Electronic Publication: 2019 Oct 23. - Publication Year :
- 2019
-
Abstract
- Previous models suggested that regulation of telomere length in Saccharomyces cerevisiae by Tel1(ATM) and Mec1(ATR) would parallel the established pathways regulating the DNA damage response. Here, we provide evidence that telomere length regulation differs from the DNA damage response in both the Tel1 and Mec1 pathways. We found that Rad53 mediates a Mec1 telomere length regulation pathway but is dispensable for Tel1 telomere length regulation, whereas in the DNA damage response, Rad53 is regulated by both Mec1 and Tel1 Using epistasis analysis with a Tel1 hypermorphic allele, Tel1-hy909, we found that the MRX complex is not required downstream of Tel1 for telomere elongation but is required downstream of Tel1 for the DNA damage response. Our data suggest that nucleolytic telomere end processing is not a required step for telomerase to elongate telomeres.<br /> (Copyright © 2019 by the Genetics Society of America.)
- Subjects :
- Amino Acid Motifs
Phosphorylation
Saccharomyces cerevisiae Proteins chemistry
Signal Transduction
Telomere Homeostasis
DNA Damage
Intracellular Signaling Peptides and Proteins metabolism
Multiprotein Complexes metabolism
Protein Serine-Threonine Kinases metabolism
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins metabolism
Telomere metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1943-2631
- Volume :
- 213
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 31645360
- Full Text :
- https://doi.org/10.1534/genetics.119.302713