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Toll-like receptor 4 regulates spontaneous intestinal tumorigenesis by up-regulating IL-6 and GM-CSF.
- Source :
-
Journal of cellular and molecular medicine [J Cell Mol Med] 2020 Jan; Vol. 24 (1), pp. 385-397. Date of Electronic Publication: 2019 Oct 25. - Publication Year :
- 2020
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Abstract
- Inflammation is as an important component of intestinal tumorigenesis. The activation of Toll-like receptor 4 (TLR4) signalling promotes inflammation in colitis of mice, but the role of TLR4 in intestinal tumorigenesis is not yet clear. About 80%-90% of colorectal tumours contain inactivating mutations in the adenomatous polyposis coli (Apc) tumour suppressor, and intestinal adenoma carcinogenesis in familial adenomatous polyposis (FAP) is also closely related to the germline mutations in Apc. The Apc <superscript>Min/+</superscript> (multiple intestinal neoplasia) model mouse is a well-utilized model of FAP, an inherited form of intestinal cancer. In this study, Apc <superscript>Min/+</superscript> intestinal adenoma mice were generated on TLR4-sufficient and TLR4-deficient backgrounds to investigate the carcinogenic effect of TLR4 in mouse gut by comparing mice survival, peripheral blood cells, bone marrow haematopoietic precursor cells and numbers of polyps in the guts of Apc <superscript>Min/+</superscript> WT and Apc <superscript>Min/+</superscript> TLR4 <superscript>-/-</superscript> mice. The results revealed that TLR4 had a critical role in promoting spontaneous intestinal tumorigenesis. Significant differential genes were screened out by the high-throughput RNA-Seq method. After combining these results with KEGG enrichment data, it was determined that TLR4 might promote intestinal tumorigenesis by activating cytokine-cytokine receptor interaction and pathways in cancer signalling pathways. After a series of validation experiments for the concerned genes, it was found that IL6, GM-CSF (CSF2), IL11, CCL3, S100A8 and S100A9 were significantly decreased in gut tumours of Apc <superscript>Min/+</superscript> TLR4 <superscript>-/-</superscript> mice compared with Apc <superscript>Min/+</superscript> WT mice. In the functional study of core down-regulation factors, it was found that IL6, GM-CSF, IL11, CCL3 and S100A8/9 increased the viability of colon cancer cell lines and decreased the apoptosis rate of colon cancer cells with irradiation and chemical treatment.<br /> (© 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.)
- Subjects :
- Adenomatous Polyposis Coli Protein metabolism
Animals
Bone Marrow Cells metabolism
Carcinogenesis genetics
Carcinogenesis pathology
Cell Line, Tumor
Disease Models, Animal
Down-Regulation genetics
Gene Expression Regulation, Neoplastic
Granulocyte-Macrophage Colony-Stimulating Factor metabolism
Hematopoietic Stem Cells metabolism
Interleukin-6 metabolism
Intestinal Polyps pathology
Mice, Inbred C57BL
Toll-Like Receptor 4 deficiency
Carcinogenesis metabolism
Granulocyte-Macrophage Colony-Stimulating Factor genetics
Interleukin-6 genetics
Intestines pathology
Toll-Like Receptor 4 metabolism
Up-Regulation genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1582-4934
- Volume :
- 24
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of cellular and molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 31650683
- Full Text :
- https://doi.org/10.1111/jcmm.14742