Back to Search
Start Over
Vascular Endothelial Growth Factor-Releasing Microspheres Based on Poly(ε-Caprolactone-PEG-ε-Caprolactone)-b-Poly(L-Lactide) Multiblock Copolymers Incorporated in a Three-Dimensional Printed Poly(Dimethylsiloxane) Cell Macroencapsulation Device.
- Source :
-
Journal of pharmaceutical sciences [J Pharm Sci] 2020 Jan; Vol. 109 (1), pp. 863-870. Date of Electronic Publication: 2019 Oct 22. - Publication Year :
- 2020
-
Abstract
- Pancreatic islet transplantation is a promising advanced therapy that has been used to treat patients suffering from diabetes type 1. Traditionally, pancreatic islets are infused via the portal vein, which is subsequently intended to engraft in the liver. Severe immunosuppressive treatments are necessary, however, to prevent rejection of the transplanted islets. Novel approaches therefore have focused on encapsulation of the islets in biomaterial implants which can protect the islets and offer an organ-like environment. Vascularization of the device's surface is a prerequisite for the survival and proper functioning of transplanted pancreatic islets. We are pursuing a prevascularization strategy by incorporation of vascular endothelial growth factor (VEGF)-loaded microspheres in 3-dimensional printed poly(dimethylsiloxane)-based devices prior to their prospective loading with transplanted cells. Microspheres (~50 μm) were based on poly(ε-caprolactone-PEG-ε-caprolactone)-b-poly(L-lactide) multiblock copolymers and were loaded with 10 μg VEGF/mg microspheres, and subsequently dispersed in a hyaluronic acid carrier liquid. In vitro release studies at 37°C demonstrated continuous release of fully bioactive VEGF for 4 weeks. In conclusion, our results demonstrate that incorporation of VEGF-releasing microspheres ensures adequate release of VEGF for a time window of 4 weeks, which is attractive in view of the vascularization of artificial pancreas implants.<br /> (Copyright © 2020 American Pharmacists Association®. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Angiogenesis Inducing Agents pharmacology
Cell Proliferation drug effects
Cells, Cultured
Drug Compounding
Drug Implants
Drug Liberation
Human Umbilical Vein Endothelial Cells drug effects
Humans
Hyaluronic Acid chemistry
Microspheres
Time Factors
Vascular Endothelial Growth Factor A pharmacology
Angiogenesis Inducing Agents chemistry
Dimethylpolysiloxanes chemistry
Drug Carriers
Polyesters chemistry
Polyethylene Glycols chemistry
Printing, Three-Dimensional
Vascular Endothelial Growth Factor A chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6017
- Volume :
- 109
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of pharmaceutical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 31654660
- Full Text :
- https://doi.org/10.1016/j.xphs.2019.10.028