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Construction of a replication-competent hepatitis B virus vector carrying secreted luciferase transgene and establishment of new hepatitis B virus replication and expression cell lines.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2019 Oct 21; Vol. 25 (39), pp. 5961-5972. - Publication Year :
- 2019
-
Abstract
- Background: Previously, we have successfully constructed replication-competent hepatitis B virus (HBV) vectors by uncoupling the P open reading frame (ORF) from the preC/C ORF to carefully design the transgene insertion site to overcome the compact organization of the HBV genome and maintain HBV replication competence. Consequently, the replication-competent HBV vectors carrying foreign genes, including pCH-BsdR, carrying blasticidin resistance gene (399 bp), and pCH-hrGFP, carrying humanized renilla green fluorescent protein gene (720 bp), were successfully obtained. However, the replication efficiency of the former is higher but it is tedious to use, while that of the latter is poor and cannot be quantified. Hence, we need to search for a new reporter gene that is convenient and quantifiable for further research.<br />Aim: To establish a helpful tool for intracellular HBV replication and anti-viral drugs screening studies.<br />Methods: We utilized the replication-competent HBV viral vectors constructed by our laboratory, combined with the secreted luciferase reporter gene, to construct replication-competent HBV vectors expressing the reporter gene secretory Nanoluc Luciferase (SecNluc). HepG2.TA2-7 cells were transfected with this vector to obtain cell lines with stably secreted HBV particles carrying secNluc reporter gene.<br />Results: The replication-competent HBV vector carrying the SecNluc reporter gene pCH-sNLuc could produce all major viral RNAs and a full set of envelope proteins and achieve high-level secreted luciferase expression. HBV replication intermediates could be produced from this vector. Via transfection with pTRE-sNLuc and selection by hygromycin, we obtained isolated cell clones, named HBV-NLuc-35 cells, which could secrete secNLuc recombinant viruses, and were sensitive to existing anti-HBV drugs. Using differentiated HepaRG cells, it was verified that recombinant HBV possessed infectivity.<br />Conclusion: Our research demonstrated that a replication-competent HBV vector carrying a secreted luciferase transgene possesses replication and expression ability, and the established HBV replication and expression cell lines could stably secrete viral particles carrying secNluc reporter gene. More importantly, the cell line and the secreted recombinant viral particles could be used to trace HBV replication or infection.<br />Competing Interests: Conflict-of-interest statement: The authors declare no conflicts of interest.<br /> (©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.)
- Subjects :
- Cell Line
Genes, Reporter genetics
Hep G2 Cells
Humans
Lamivudine pharmacology
Microbial Sensitivity Tests methods
Plasmids genetics
RNA genetics
RNA, Viral genetics
Transfection methods
Transgenes genetics
Virus Replication genetics
Antiviral Agents pharmacology
Genetic Vectors genetics
Hepatitis B virus genetics
Luciferases genetics
Virus Replication drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 2219-2840
- Volume :
- 25
- Issue :
- 39
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 31660033
- Full Text :
- https://doi.org/10.3748/wjg.v25.i39.5961