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Isolation and antibiotic sensitivity pattern of drug resistant bacteria in ulcerative foot of type 2 diabetic patients.
- Source :
-
Pakistan journal of pharmaceutical sciences [Pak J Pharm Sci] 2019 Jul; Vol. 32 (4 (Suppl)), pp. 1843-1848. - Publication Year :
- 2019
-
Abstract
- The present study aimed to decipher the bacterial infections in diabetic foot human patients in Pakistan and the anti-microbial susceptibility for clinical relevance. A total of 30 samples were collected from hospitalized type 2 diabetic patients (men and women) having foot ulcers. The collected samples were cultured on mannitol salt agar, Blood agar and MacConkey's agar and cetrimide agar. Gram staining and specific biochemical tests were performed to identify the invading bacteria. Antibiotic sensitivity and resistance pattern was performed for isolated bacteria by Kirby-Bauer disc diffusion method. In diabetic foot ulcers, most prevalent bacteria were S. aureus with percent positivity of 83% followed by E. coli (66%), K. pneumoniae (40%) and P. aeruginosa (16%). The infected ulcer with poly-microorganisms was 83.4% and the infected ulcer with single isolates was 16.6%. Imipenem was found to be most sensitive antibiotic against Gram positive as well as Gram negative bacterial isolates from diabetic foot ulcer human patients. Gram negative isolates from diabetic foot showed resistance to ampicillin, sulfamethoxazole/trimethoprim, cefotaxime/clavulanate, metronidazole. The diabetic foot ulcers of human patients revealed high prevalence of S. aureus followed by E. coli, K. pneumoniae and P. aeruginosa respectively. Imipenem was found to be the most sensitive antibiotic for all the bacterial isolates from foot ulcers of type 2 diabetic patients. This study suggests imipenem as effective antibiotic for treatment of diabetic foot ulcers against bacteria.
- Subjects :
- Diabetes Mellitus, Type 2 complications
Diabetic Foot etiology
Female
Humans
Male
Microbial Sensitivity Tests methods
Middle Aged
Pakistan
Anti-Bacterial Agents therapeutic use
Bacteria drug effects
Bacteria isolation & purification
Diabetes Mellitus, Type 2 microbiology
Diabetic Foot drug therapy
Diabetic Foot microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1011-601X
- Volume :
- 32
- Issue :
- 4 (Suppl)
- Database :
- MEDLINE
- Journal :
- Pakistan journal of pharmaceutical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 31680081