Back to Search
Start Over
Cryogenic Ion Spectroscopy of the Green Fluorescent Protein Chromophore in Vacuo.
- Source :
-
The journal of physical chemistry letters [J Phys Chem Lett] 2019 Dec 19; Vol. 10 (24), pp. 7817-7822. Date of Electronic Publication: 2019 Dec 05. - Publication Year :
- 2019
-
Abstract
- We present the spectrum of the S <subscript>1</subscript> ← S <subscript>0</subscript> transition of an anionic model for the chromophore of the green fluorescent protein in vacuo at cryogenic temperatures, showing previously unresolved vibrational features, and resolving the band origin at 20 930 cm <superscript>-1</superscript> (477.8 nm) with unprecedented accuracy. The vibrational spectrum establishes that the molecule is in the Z isomer at low temperature. At increased temperature, the S <subscript>1</subscript> ← S <subscript>0</subscript> band shifts to the red, which we tentatively attribute to emergent population of the E isomer.
- Subjects :
- Cold Temperature
Ions chemistry
Isomerism
Nitrogen chemistry
Photochemical Processes
Protein Conformation
Solvents chemistry
Spectrometry, Fluorescence methods
Thermodynamics
Vibration
Benzylidene Compounds chemistry
Fluorescent Dyes chemistry
Green Fluorescent Proteins chemistry
Imidazoles chemistry
Models, Molecular
Subjects
Details
- Language :
- English
- ISSN :
- 1948-7185
- Volume :
- 10
- Issue :
- 24
- Database :
- MEDLINE
- Journal :
- The journal of physical chemistry letters
- Publication Type :
- Academic Journal
- Accession number :
- 31682445
- Full Text :
- https://doi.org/10.1021/acs.jpclett.9b02916