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A chromosome folding intermediate at the condensin-to-cohesin transition during telophase.
- Source :
-
Nature cell biology [Nat Cell Biol] 2019 Nov; Vol. 21 (11), pp. 1393-1402. Date of Electronic Publication: 2019 Nov 04. - Publication Year :
- 2019
-
Abstract
- Chromosome folding is modulated as cells progress through the cell cycle. During mitosis, condensins fold chromosomes into helical loop arrays. In interphase, the cohesin complex generates loops and topologically associating domains (TADs), while a separate process of compartmentalization drives segregation of active and inactive chromatin. We used synchronized cell cultures to determine how the mitotic chromosome conformation transforms into the interphase state. Using high-throughput chromosome conformation capture (Hi-C) analysis, chromatin binding assays and immunofluorescence, we show that, by telophase, condensin-mediated loops are lost and a transient folding intermediate is formed that is devoid of most loops. By cytokinesis, cohesin-mediated CTCF-CTCF loops and the positions of TADs emerge. Compartment boundaries are also established early, but long-range compartmentalization is a slow process and proceeds for hours after cells enter G1. Our results reveal the kinetics and order of events by which the interphase chromosome state is formed and identify telophase as a critical transition between condensin- and cohesin-driven chromosome folding.
- Subjects :
- Adenosine Triphosphatases metabolism
Cell Compartmentation genetics
Cell Cycle Proteins metabolism
Cell Line, Transformed
Chromatin ultrastructure
Chromosomal Proteins, Non-Histone metabolism
Chromosome Mapping
Cytokinesis genetics
DNA-Binding Proteins metabolism
Gene Expression
HeLa Cells
Humans
Interphase
Multiprotein Complexes metabolism
S Phase
Cohesins
Adenosine Triphosphatases genetics
Cell Cycle Proteins genetics
Chromatin metabolism
Chromosomal Proteins, Non-Histone genetics
DNA-Binding Proteins genetics
Multiprotein Complexes genetics
Telophase
Subjects
Details
- Language :
- English
- ISSN :
- 1476-4679
- Volume :
- 21
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Nature cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 31685986
- Full Text :
- https://doi.org/10.1038/s41556-019-0406-2