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siRNA-mediated knockdown of sperm-associated antigen 11a (Spag11a) mRNA in epididymal primary epithelial cells affects proliferation: a transcriptome analyses.
- Source :
-
Cell and tissue research [Cell Tissue Res] 2020 Mar; Vol. 379 (3), pp. 601-612. Date of Electronic Publication: 2019 Nov 05. - Publication Year :
- 2020
-
Abstract
- Differential expression of a variety of proteins in the four major regions of the epididymis contributes to maturation of spermatozoa and region-specific cellular functions as well. Proliferation of epithelial cells of the epididymis is highly controlled and thus is one of the major reasons for the nonoccurrence of cancers in this organ system. The molecular mechanisms and the contribution of region-specific genes in epithelial cell proliferation are not yet fully understood. In this study, for the first time, we analyzed the role of sperm-associated antigen 11a (Spag11a), a caput-specific beta-defensin-like antimicrobial gene in governing epididymal cell proliferation and global gene expression. siRNA-mediated knockdown of Spag11a mRNA in epididymal primary epithelial cells resulted in increased cell proliferation. Out of the 68,842 genes analyzed, 4182 genes were differentially expressed (2154 upregulated and 2028 downregulated). A variety of genes that participate in different cellular processes and pathways were differentially regulated. Genes that are important for epithelial cell proliferation were found to be differentially regulated and these changes were confirmed by real-time PCR. Overexpression of Spag11a in immortalized rat caput epididymal cells resulted in decreased proliferation capacity. Results of this study indicate that Spag11a plays a crucial role in governing epididymal epithelial cell proliferation.
- Subjects :
- Animals
Blotting, Western
Cell Proliferation physiology
Epididymis cytology
Epididymis metabolism
Gene Expression Profiling
Gene Knockdown Techniques
Lipids administration & dosage
Male
RNA, Messenger antagonists & inhibitors
RNA, Messenger biosynthesis
RNA, Messenger genetics
RNA, Messenger metabolism
RNA, Small Interfering administration & dosage
RNA, Small Interfering genetics
Rats
Rats, Wistar
beta-Defensins genetics
beta-Defensins metabolism
Epididymis physiology
Epithelial Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0878
- Volume :
- 379
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cell and tissue research
- Publication Type :
- Academic Journal
- Accession number :
- 31691005
- Full Text :
- https://doi.org/10.1007/s00441-019-03107-6