Utilization of oil palm decanter cake for valuable laccase and manganese peroxidase enzyme production from a novel white-rot fungus, Pseudolagarobasidium sp. PP17-33.

Oil palm decanter cake (OPDC) in the current study was converted to valuable products as laccase and manganese peroxidase (MnP) by an undescribed strain of the white-rot fungus, Pseudolagarobasidium sp. PP17-33. The optimization to enhance the production of enzymes through solid-state fermentation was performed using Plackett-Burman design and response surface methodology. The highest observed laccase was 5.841 U/gds and observed MnP was 5.156 U/gds, which enhanced yield by 2.59-fold and 1.94-fold from the non-optimization. The optimized medium (mg/g of OPDC) consisted of 0.852 mg CuSO ₄ ·5H ₂ O, 13.512 mg glucose, 2 mg yeast extract, 0.2 mg KH ₂ PO ₄ , 1.5 mg MgSO ₄ ·7H ₂ O, 0.01 mg FeSO ₄ ·7H ₂ O, 0.15 mg MnSO ₄ ·H ₂ O, 0.01 mg ZnSO ₄ ·7H ₂ O and 0.3 mg Tween 80 (pH 5.0) when incubated at 30 °C for 7 days. The most significant variables of laccase and MnP productions were CuSO ₄ ·5H ₂ O and glucose concentrations. This study is the first to report on the production of ligninolytic enzymes from OPDC waste using white-rot fungi. In addition, five different white-rot fungi, Coriolopsis aspera , C. retropicta , Dentipellis parmastoi , Nigroporus vinosus and Tyromyces xuchilensis, are newly observed producers of ligninolytic enzymes in Thailand. The results obtained from this study are significant not only for agro-industrial waste management but also for value-added enzyme production.
Competing Interests: Conflict of interestThe authors declared no conflict of interest.
(© King Abdulaziz City for Science and Technology 2019.)