Flavobacterium psychrotolerans sp. nov., a psychrotolerant bacterium isolated from Renlongba glacier on the Tibetan Plateau.

One aerobic, Gram-stain-negative, rod-shaped bacterium, designated strain RB1R5 ^T , was isolated from Renlongba glacier in Tibet Autonomous Region, China. Growth was observed at 4-25 °C and pH 7.0-8.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RB1R5 ^T belongs to the genus Flavobacterium , and its closest relatives are Flavobacterium luteum IMCC26026 ^T and Flavobacterium psychroterrae CCM 8827 ^T with 97.75 and 97.42% gene sequence similarities, respectively. The average nucleotide identity and DNA-DNA hybridization values between strain RB1R5 ^T and its closest relative Flavobacterium luteum IMCC26026 ^T were 78.02 and 21.8 %, respectively. The major cellular fatty acids were anteiso-C _15 : 0 and summed feature 3 (comprising C _16:1 ω7 c /C _16:1 ω6 c ). The sole menaquinone was MK-6. The major phospholipid was phosphatidylethanolamine. On the basis of phenotypic, phylogenetic and chemotaxonomic characterizations, strain RB1R5 ^T represents a novel species of the genus Flavobacterium , for which the name Flavobacterium psychrotolerans sp. nov. is proposed. The type strain is RB1R5 ^T (=CGMCC 1.23024 ^T =NBRC 113060 ^T ).