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Engineering cytoplasmic acetyl-CoA synthesis decouples lipid production from nitrogen starvation in the oleaginous yeast Rhodosporidium azoricum.
- Source :
-
Microbial cell factories [Microb Cell Fact] 2019 Nov 14; Vol. 18 (1), pp. 199. Date of Electronic Publication: 2019 Nov 14. - Publication Year :
- 2019
-
Abstract
- Background: Oleaginous yeasts are able to accumulate very high levels of neutral lipids especially under condition of excess of carbon and nitrogen limitation (medium with high C/N ratio). This makes necessary the use of two-steps processes in order to achieve high level of biomass and lipid. To simplify the process, the decoupling of lipid synthesis from nitrogen starvation, by establishing a cytosolic acetyl-CoA formation pathway alternative to the one catalysed by ATP-citrate lyase, can be useful.<br />Results: In this work, we introduced a new cytoplasmic route for acetyl-CoA (AcCoA) formation in Rhodosporidium azoricum by overexpressing genes encoding for homologous phosphoketolase (Xfpk) and heterologous phosphotransacetylase (Pta). The engineered strain PTAPK4 exhibits higher lipid content and produces higher lipid concentration than the wild type strain when it was cultivated in media containing different C/N ratios. In a bioreactor process performed on glucose/xylose mixture, to simulate an industrial process for lipid production from lignocellulosic materials, we obtained an increase of 89% in final lipid concentration by the engineered strain in comparison to the wild type. This indicates that the transformed strain can produce higher cellular biomass with a high lipid content than the wild type. The transformed strain furthermore evidenced the advantage over the wild type in performing this process, being the lipid yields 0.13 and 0.05, respectively.<br />Conclusion: Our results show that the overexpression of homologous Xfpk and heterologous Pta activities in R. azoricum creates a new cytosolic AcCoA supply that decouples lipid production from nitrogen starvation. This metabolic modification allows improving lipid production in cultural conditions that can be suitable for the development of industrial bioprocesses using lignocellulosic hydrolysates.
- Subjects :
- Acetyl Coenzyme A metabolism
Aldehyde-Lyases genetics
Aldehyde-Lyases metabolism
Bacillus subtilis genetics
Biomass
Cytoplasm metabolism
Fungal Proteins genetics
Genes, Bacterial
Genes, Fungal
Genetic Engineering
Homologous Recombination
Lipid Metabolism genetics
Nitrogen metabolism
Phosphate Acetyltransferase genetics
Phosphate Acetyltransferase metabolism
Recombinant Proteins
Transfection
Basidiomycota metabolism
Lignin metabolism
Lipids biosynthesis
Metabolic Engineering methods
Subjects
Details
- Language :
- English
- ISSN :
- 1475-2859
- Volume :
- 18
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Microbial cell factories
- Publication Type :
- Academic Journal
- Accession number :
- 31727065
- Full Text :
- https://doi.org/10.1186/s12934-019-1250-6